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https://hdl.handle.net/2440/115682
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Type: | Journal article |
Title: | Non-canonical function of spindle assembly checkpoint proteins after APC activation reduces aneuploidy in mouse oocytes |
Author: | Lane, S. Jones, K. |
Citation: | Nature Communications, 2014; 5(1):3444-1-3444-9 |
Publisher: | Springer Nature |
Issue Date: | 2014 |
ISSN: | 2041-1723 2041-1723 |
Statement of Responsibility: | Simon I.R. Lane and Keith T. Jones |
Abstract: | The spindle assembly checkpoint (SAC) prevents aneuploidy by coupling anaphase onset, through anaphase-promoting complex (APC) activation, with chromosome attachment to spindle microtubules. Here, we examine APC activity in oocytes, noted for their susceptibility to chromosome mis-segregation during the first meiotic division (MI). We find that MI oocytes only contain sub-maximal APC activity, measured through cyclin B1-GFP degradation, because inhibition of SAC proteins when the APC is normally fully active increases cyclin B1 degradation twofold and reduces the length of this division by 2 h. In addition, inhibiting the SAC component Mps1 only when the APC is already active increases aneuploidy rates in the resulting egg by up to 30%. We therefore establish that the activities of SAC proteins and the APC co-exist in oocytes, and such concurrence has a vital role in reducing aneuploidy rates by extending MI, probably by allowing time for numerous erroneous microtubule attachments to be corrected. |
Keywords: | Oocytes |
Description: | Published 18 Mar 2014 |
Rights: | © 2014 Macmillan Publishers Limited. All rights reserved. |
DOI: | 10.1038/ncomms4444 |
Grant ID: | http://purl.org/au-research/grants/arc/DP110100418 http://purl.org/au-research/grants/arc/DP120100946 |
Published version: | http://dx.doi.org/10.1038/ncomms4444 |
Appears in Collections: | Aurora harvest 8 Molecular and Biomedical Science publications |
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