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Type: Journal article
Title: Non-canonical function of spindle assembly checkpoint proteins after APC activation reduces aneuploidy in mouse oocytes
Author: Lane, S.
Jones, K.
Citation: Nature Communications, 2014; 5(1):3444-1-3444-9
Publisher: Springer Nature
Issue Date: 2014
ISSN: 2041-1723
Statement of
Simon I.R. Lane and Keith T. Jones
Abstract: The spindle assembly checkpoint (SAC) prevents aneuploidy by coupling anaphase onset, through anaphase-promoting complex (APC) activation, with chromosome attachment to spindle microtubules. Here, we examine APC activity in oocytes, noted for their susceptibility to chromosome mis-segregation during the first meiotic division (MI). We find that MI oocytes only contain sub-maximal APC activity, measured through cyclin B1-GFP degradation, because inhibition of SAC proteins when the APC is normally fully active increases cyclin B1 degradation twofold and reduces the length of this division by 2 h. In addition, inhibiting the SAC component Mps1 only when the APC is already active increases aneuploidy rates in the resulting egg by up to 30%. We therefore establish that the activities of SAC proteins and the APC co-exist in oocytes, and such concurrence has a vital role in reducing aneuploidy rates by extending MI, probably by allowing time for numerous erroneous microtubule attachments to be corrected.
Keywords: Oocytes
Description: Published 18 Mar 2014
Rights: © 2014 Macmillan Publishers Limited. All rights reserved.
RMID: 0030095276
DOI: 10.1038/ncomms4444
Grant ID:
Appears in Collections:Molecular and Biomedical Science publications

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