Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/115683
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Type: Journal article
Title: Premature dyad separation in meiosis II is the major segregation error with maternal age in mouse oocytes
Author: Yun, Y.
Lane, S.
Jones, K.
Citation: Development, 2014; 141(1):199-208
Publisher: Company of Biologists
Issue Date: 2014
ISSN: 0950-1991
1477-9129
Statement of
Responsibility: 
Yan Yun, Simon I. R. Lane and Keith T. Jones
Abstract: As women get older their oocytes become susceptible to chromosome mis-segregation. This generates aneuploid embryos, leading to increased infertility and birth defects. Here we examined the provenance of aneuploidy by tracking chromosomes and their kinetochores in oocytes from young and aged mice. Changes consistent with chromosome cohesion deterioration were found with age, including increased interkinetochore distance and loss of the centromeric protector of cohesion SGO2 in metaphase II arrested (metII) eggs, as well as a rise in the number of weakly attached bivalents in meiosis I (MI) and lagging chromosomes at anaphase I. However, there were no MI errors in congression or biorientation. Instead, premature separation of dyads in meiosis II was the major segregation defect in aged eggs and these were associated with very low levels of SGO2. These data show that although considerable cohesion loss occurs during MI, its consequences are observed during meiosis II, when centromeric cohesion is needed to maintain dyad integrity.
Keywords: Imaging; Chromosomes; Meiosis; Mouse; Oocytes
Rights: © 2014. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
RMID: 0030095278
DOI: 10.1242/dev.100206
Grant ID: http://purl.org/au-research/grants/arc/DP110100418
http://purl.org/au-research/grants/arc/DP120100946
Appears in Collections:Molecular and Biomedical Science publications

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