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Type: Journal article
Title: Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance and no yield penalty under controlled growth conditions
Author: Bi, H.
Shi, J.
Kovalchuk, N.
Luang, S.
Bazanova, N.
Chirkova, L.
Zhang, D.
Shavrukov, Y.
Stepanenko, A.
Tricker, P.
Langridge, P.
Hrmova, M.
Lopato, S.
Borisjuk, N.
Citation: Plant, Cell & Environment, 2018; 41(11):2549-2566
Publisher: Wiley
Issue Date: 2018
ISSN: 0140-7791
Statement of
Huihui Bi, Jianxin Shi, Nataliya Kovalchuk, Sukanya Luang, Natalia Bazanova, Larissa Chirkova, Dabing Zhang, Yuri Shavrukov, Anton Stepanenko, Penny Tricker, Peter Langridge, Maria Hrmova, Sergiy Lopato, Nikolai Borisjuk
Abstract: Transcription factors regulate multiple networks, mediating the responses of organisms to stresses, including drought. Here we investigated the role of the wheat transcription factor TaSHN1 in crop growth and drought tolerance. TaSHN1, isolated from bread wheat, was characterised for molecular interactions and functionality. The overexpression of TaSHN1 in wheat was followed by the evaluation of T2 and T3 transgenic lines for drought tolerance, growth and yield components. Leaf surface changes were analysed by light microscopy, SEM, TEM and GC-MS/GC-FID. TaSHN1 behaves as a transcriptional activator in a yeast transactivation assay and binds stress-related DNA cis-elements, determinants of which were revealed using 3D molecular modelling. The overexpression of TaSHN1 in transgenic wheat did not result in a yield penalty under the controlled plant growth conditions of a glasshouse. Transgenic lines had significantly lower stomatal density and leaf water loss, and exhibited improved recovery after severe drought, compared to control plants. The comparative analysis of cuticular waxes revealed an increased accumulation of alkanes in leaves of transgenic lines. Our data demonstrate that TaSHN1 may operate as a positive modulator of drought stress tolerance. Positive attributes could be mediated through an enhanced accumulation of alkanes and reduced stomatal density.
Keywords: 3D molecular modelling; cuticle; stomata; transgenic wheat; water loss
Rights: © 2018 John Wiley & Sons Ltd
RMID: 0030086915
DOI: 10.1111/pce.13339
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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