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Type: Journal article
Title: Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species
Author: Ansari, T.
Bertozzi, T.
Hacking, J.
Cooper, S.
Gardner, M.
Citation: Australian Journal of Zoology, 2014; 62(6):515-518
Publisher: CSIRO Publishing
Issue Date: 2014
ISSN: 0004-959X
Statement of
Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper and Michael G. Gardner
Abstract: We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a ‘northern’ lineage (48 loci) and a ‘southern’ lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.
Keywords: DNA markers; phylogeography; squamate
Rights: Journal compilation © CSIRO 2014
DOI: 10.1071/ZO14085
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Appears in Collections:Agriculture, Food and Wine publications
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