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Type: Journal article
Title: Enhanced amplification of hepatitis C virus (HCV) cDNA by PCR: Detection of HCV RNA in archival sera
Author: Beardsley, A.
Gowans, E.
Burrell, C.
Marmion, B.
Citation: Journal of Clinical Microbiology, 1996; 34(6):1581-1582
Issue Date: 1996
ISSN: 0095-1137
Statement of
A M Beardsley, E J Gowans, C J Burrell and B P Marmion
Abstract: A reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral genome.
Keywords: Humans
Moloney murine leukemia virus
Hepatitis C
RNA-Directed DNA Polymerase
DNA, Complementary
RNA, Viral
Sensitivity and Specificity
Polymerase Chain Reaction
Base Sequence
Evaluation Studies as Topic
DOI: 10.1128/jcm.34.6.1581-1582.1996
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Appears in Collections:Aurora harvest 7
Microbiology and Immunology publications

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