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Type: Journal article
Title: A rice Ca²⁺ binding protein is required for tapetum function and pollen formation
Other Titles: A rice Ca(2+) binding protein is required for tapetum function and pollen formation
Author: Yu, J.
Meng, Z.
Liang, W.
Behera, S.
Kudla, J.
Tucker, M.
Luo, Z.
Chen, M.
Xu, D.
Zhao, G.
Wang, J.
Zhang, S.
Kim, Y.
Zhang, D.
Citation: Plant Physiology, 2016; 172(3):1772-1786
Publisher: American Society of Plant Biologists
Issue Date: 2016
ISSN: 0032-0889
Statement of
Jing Yu, Zhaolu Meng, Wanqi Liang, Smrutisanjita Behera, Jörg Kudla, Matthew R. Tucker, Zhijing Luo, Mingjiao Chen, Dawei Xu, Guochao Zhao, Jie Wang, Siyi Zhang, Yu-Jin Kim, Dabing Zhang
Abstract: In flowering plants, successful male reproduction requires the sophisticated interaction between somatic anther wall layers and reproductive cells. Timely degradation of the innermost tissue of the anther wall layer, the tapetal layer, is critical for pollen development. Ca2+ is a well-known stimulus for plant development, but whether it plays a role in affecting male reproduction remains elusive. Here we report a role of Defective in Exine Formation 1 (OsDEX1) in rice (Oryza sativa), a Ca2+ binding protein, in regulating rice tapetal cell degradation and pollen formation. In osdex1 anthers, tapetal cell degeneration is delayed and degradation of the callose wall surrounding the microspores is compromised, leading to aborted pollen formation and complete male sterility. OsDEX1 is expressed in tapetal cells and microspores during early anther development. Recombinant OsDEX1 is able to bind Ca2+ and regulate Ca2+ homeostasis in vitro, and osdex1 exhibited disturbed Ca2+ homeostasis in tapetal cells. Phylogenetic analysis suggested that OsDEX1 may have a conserved function in binding Ca2+ in flowering plants, and genetic complementation of pollen wall defects of an Arabidopsis (Arabidopsis thaliana) dex1 mutant confirmed its evolutionary conservation in pollen development. Collectively, these findings suggest that OsDEX1 plays a fundamental role in the development of tapetal cells and pollen formation, possibly via modulating the Ca2+ homeostasis during pollen development.
Keywords: Plants, Genetically Modified; Pollen; Calcium-Binding Proteins; Plant Proteins; Recombinant Proteins; Cloning, Molecular; Phylogeny; Cell Death; DNA Fragmentation; Gene Expression Regulation, Plant; Homeostasis; Phenotype; Mutation; Models, Biological; Oryza
Rights: © 2016 American Society of Plant Biologists. All Rights Reserved.
RMID: 0030056090
DOI: 10.1104/pp.16.01261
Appears in Collections:Agriculture, Food and Wine publications

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