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https://hdl.handle.net/2440/11627
Type: | Journal article |
Title: | Granulocyte-macrophage colony-stimulating factor enhances EBV-induced synthesis of chemotactic factors in human neutrophils. |
Author: | Roberge, C. McColl, S. Larochelle, B. Gosselin, J. |
Citation: | Journal of Immunology, 1998; 160(5):2442-2448 |
Publisher: | AMER ASSOC IMMUNOLOGISTS |
Issue Date: | 1998 |
ISSN: | 0022-1767 1550-6606 |
Abstract: | We have recently demonstrated that EBV binds to human neutrophils and stimulates a wide range of activities, including homeotypic aggregation, total RNA synthesis, and expression of the chemokines IL-8 and macrophage inflammatory protein-1alpha (MIP-1alpha). Neutrophil function is also known to be modulated by priming with granulocyte-macrophage colony-stimulating factor (GM-CSF). We have therefore investigated the modulation of EBV-induced activation of human neutrophils by GM-CSF. Treatment of neutrophils with GM-CSF before EBV activation enhanced the production of both MIP-1alpha and IL-8. The IL-8 produced under these conditions was biologically active as determined in the calcium mobilization assay. GM-CSF was also found to increase the ability of EBV to prime neutrophils for increased leukotriene B4 (LTB4) synthesis. Prior treatment of GM-CSF with neutralizing Abs inhibited these effects. GM-CSF also increased the specific binding of FITC-EBV to the neutrophil surface, as evaluated by fluorocytometry. Local production of GM-CSF in tissues invaded by EBV could therefore serve to potentiate a host defense mechanism directed toward the destruction of the infectious virus via increased production of chemotactic factors. Since both IL-8 and MIP-1alpha are reported to be chemoattractants in vitro for T cells and T and B cells, respectively, the ability of EBV to induce their production by neutrophils may enhance its ability to infect B and T lymphocytes via increased recruitment to sites of infection. |
Keywords: | Neutrophils Humans Herpesvirus 4, Human Calcium Leukotriene B4 Granulocyte-Macrophage Colony-Stimulating Factor RNA, Messenger Interleukin-8 Macrophage Inflammatory Proteins Chemotactic Factors Adjuvants, Immunologic Neutrophil Activation Chemokine CCL3 Chemokine CCL4 |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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