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Type: Journal article
Title: Effects of TNF-a, IL-1b and activated peripheral blood mononuclear cells on the expression of adhesion molecules and recruitment of leukocytes in rheumatoid synovial xenografts in SCID mice.
Author: Proudman, S.
Cleland, L.
Mayrhofer, G.
Citation: Journal of Rheumatology, 1999; 26(9):1877-1889
Issue Date: 1999
ISSN: 0315-162X
Abstract: <h4>Objective</h4>To examine expression of adhesion molecules within human synovial xenografts in vivo after injection of human cytokines or peripheral blood mononuclear cells (PBMC).<h4>Methods</h4>Rheumatoid synovium was transplanted into subcutaneous pouches in the ears of severe combined immunodeficient (SCID) mice. Tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), or PBMC were injected into the xenografts. The grafts were assessed by immunohistochemistry and quantitative video image analysis.<h4>Results</h4>TNF-alpha, IL-1beta, and concanavalin A activated PBMC increased the expression of vascular cellular adhesion molecule 1 and intercellular adhesion molecule 1 significantly on vessels and nonvascular synovial cells compared with injection of medium only. Vascular expression of E-selectin, but not of MHC Class II, was also increased. These stimuli also induced the migration of murine leukocytes into the xenografts.<h4>Conclusion</h4>Rheumatoid synovial xenografts in SCID mice respond by upregulation of adhesion molecule expression when challenged in vivo with proinflammatory cytokines or activated PBMC.
Keywords: Synovial Membrane
Leukocytes, Mononuclear
Mice, SCID
Arthritis, Rheumatoid
Disease Models, Animal
Tumor Necrosis Factor-alpha
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
Transplantation, Heterologous
Injections, Intralesional
Sensitivity and Specificity
Cell Movement
Appears in Collections:Aurora harvest 7
Microbiology and Immunology publications

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