Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Photoswitchable peptide-based ‘on-off’ biosensor for electrochemical detection and control of protein-protein interactions|
|Citation:||Biosensors and Bioelectronics, 2018; 118:188-194|
|John R.Horsley, Jingxian Yu, Kate L.Wegener, Christian Hoppmann, Karola Rück-Braun, Andrew D.Abell|
|Abstract:||Neuronal nitric oxide synthase (nNOS) is an enzyme responsible for catalyzing the production of the crucial cellular signalling molecule, nitric oxide (NO), through its interaction with the PDZ domain of α-syntrophin protein. In this study, a novel light-driven photoswitchable peptide-based biosensor, modelled on the nNOS β-finger, is used to detect and control its interaction with α-syntrophin. An azobenzene photoswitch incorporated into the peptide backbone allows reversible switching between a trans photostationary state devoid of secondary structure, and a cis photostationary state possessing a well-defined antiparallel β-strand geometry, as revealed by molecular modelling. Electrochemical impedance spectroscopy (EIS) is used to successfully detect the interaction between the gold electrode bound peptide in its cis photostationary state and a wide range of concentrations of α-syntrophin protein, highlighting both the qualitative and quantitative properties of the sensor. Furthermore, EIS demonstrates that the probe in its random trans photostationary state does not bind to the target protein. The effectiveness of the biosensor is further endorsed by the high thermal stability of the photostationary state of the cis-isomer, and the ability to actively control biomolecular interactions using light. This approach allows detection and control of binding to yield a regenerable on-off biosensor.|
|Keywords:||Biosensors; Electrochemical impedance spectroscopy; Neuronal nitric oxide synthase (nNOS); Photoswitchable peptides; Protein-protein interaction; Secondary structure|
|Rights:||© 2018 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Physics publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.