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|Title:||A rotating light cycle promotes weight gain and hepatic lipid storage in mice|
|Citation:||American Journal of Physiology - Gastrointestinal and Liver Physiology, 2018; 315(6):932-941|
|Publisher:||American Physiological Society|
|Stewart Christie, Andrew D. Vincent, Hui Li, Claudine L. Frisby, Stephen J. Kentish, Rebecca O’Rielly, Gary A. Wittert and Amanda J. Page|
|Abstract:||Processes involved in regulation of energy balance and intermediary metabolism are aligned to the light-dark cycle. Shift-work and high-fat diet (HFD)-induced obesity disrupt circadian rhythmicity and are associated with increased risk of nonalcoholic fatty liver disease. This study aimed to determine the effect of simulating shift work on hepatic lipid accumulation in lean and HFD mice. C57BL/6 mice fed a standard laboratory diet (SLD) or HFD for 4 wk were further allocated to a normal light (NL) cycle (lights on: 0600–1800) or rotating light (RL) cycle [3 days NL and 4 days reversed (lights on: 1800–0600) repeated] for 8 wk. Tissue was collected every 3 h beginning at 0600. HFD mice gained more weight than SLD mice, and RL mice gained more weight than NL mice. SLD-NL and HFD-NL mice, but not RL mice, were more active, had higher respiratory quotients, and consumed/expended more energy during the dark phase compared with the light phase. Blood glucose and plasma cholesterol and triglyceride concentrations were elevated in HFD and SLD-RL compared with SLD-NL mice. Hepatic glycogen was elevated in HFD compared with SLD mice. Hepatic triglycerides were elevated in SLD-RL and HFD mice compared with SLD-NL. Circadian rhythmicity of hepatic acetyl-CoA carboxylase (ACACA) mRNA was phase shifted in SLD-RL and HFD-NL and lost in HFD-RL mice. Hepatic ACACA protein was reduced in SLD-RL and HFD mice compared with SLD-NL mice. Hepatic adipose triglyceride lipase was elevated in HFD-NL compared with SLD-NL but lower in RL mice compared with NL mice irrespective of diet. In conclusion, an RL cycle model of shift work promotes weight gain and hepatic lipid storage even in lean conditions.|
|Keywords:||Fatty liver; high-fat diet; metabolism; rotating light cycle|
|Rights:||© 2018 the American Physiological Society. All rights reserved.|
|Appears in Collections:||Physiology publications|
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