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Type: Journal article
Title: Defective tapetum cell death 1 (DTC1) regulates ROS levels by binding to metallothionein during tapetum degeneration
Author: Yi, J.
Moon, S.
Lee, Y.
Zhu, L.
Liang, W.
Zhang, D.
Jung, K.
An, G.
Citation: Plant Physiology, 2016; 170(3):1611-1623
Publisher: American Society of Plant Biologists
Issue Date: 2016
ISSN: 0032-0889
Statement of
Jakyung Yi, Sunok Moon, Yang-Seok Lee, Lu Zhu, Wanqi Liang, Dabing Zhang, Ki-Hong Jung, and Gynheung An
Abstract: After meiosis, tapetal cells in the innermost anther wall layer undergo program cell death (PCD)-triggered degradation. This step is essential for microspore development and pollen wall maturation. We identified a key gene, Defective Tapetum Cell Death 1 (DTC1), that controls this degeneration by modulating the dynamics of reactive oxygen species (ROS) during rice male reproduction. Mutants defective in DTC1 exhibit phenotypes of an enlarged tapetum and middle layer with delayed degeneration, causing male sterility. The gene is preferentially expressed in the tapetal cells during early anther development. In dtc1 anthers, expression of genes encoding secretory proteases or lipid transporters is significantly reduced, while transcripts of PCD regulatory genes, e.g. UDT1, TDR1, and EAT1/DTD, are not altered. Moreover, levels of DTC1 transcripts are diminished in udt1, tdr, and eat1 anthers. These results suggest that DTC1 functions downstream of those transcription factor genes and upstream of the genes encoding secretory proteins. DTC1 protein interacts with OsMT2b, a ROS scavenger. Whereas wild-type plants accumulate large amounts of ROS in their anthers at Stage 9 of development, those levels remain low during all stages of development in dtc1 anthers. These findings indicate that DTC1 is a key regulator for tapetum PCD by inhibiting ROS-scavenging activity.
Keywords: Plant modification
Rights: © 2016 American Society of Plant Biologists. All Rights Reserved.
RMID: 0030049706
DOI: 10.1104/pp.15.01561
Appears in Collections:Agriculture, Food and Wine publications

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