Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11689
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Type: Journal article
Title: Rapid and efficient cell-to-cell transmission of human immunodeficiency virus infection from monocyte-derived macrophages to peripheral blood lymphocytes
Author: Carr, J.
Hocking, H.
Li, P.
Burrell, C.
Citation: Virology, 1999; 265(2):319-329
Publisher: ACADEMIC PRESS INC
Issue Date: 1999
ISSN: 0042-6822
1096-0341
Abstract: Macrophages are considered of central importance in cell-to-cell transmission of human immunodeficiency virus (HIV) infection in vivo. In this report, we describe a novel cell-to-cell transmission model using HIV-infected monocyte-derived macrophages (MDMs) as donor cells and peripheral blood lymphocytes (PBLs) as recipients. Virus was transmitted during a 2-h coincubation period from intracellular or tightly cell-associated viral stores in adherent infected MDMs to nonadherent CD3(+) PBLs. Transmission required cell contact, but syncytia formation was not observed. HIV cell-to-cell transmission occurred in both allogeneic and autologous systems, and replication was higher in phytohemagglutinin (PHA)-stimulated than unstimulated recipient PBLs. In contrast, transmission of infection by cell-free virus was barely detectable without PHA stimulation of recipients, suggesting the cell-cell interaction may have provided stimuli to recipient cells in the cell-to-cell system. Viral DNA levels increased 5-24 h postmixing, and this increase was inhibited by pretreatment of cells with the reverse transcription inhibitor azidothymidine, indicating de novo reverse transcription was involved. Cell-to-cell transmission was more efficient than infection with cell-free virus released from donor MDMs, or 0.1 TCID(50)/cell cell-free viral challenge. This model provides a system to further investigate the mechanisms and characteristics of HIV cell-to-cell transmission between relevant primary cells that may be analogous to this important mode of virus spread in vivo.
Keywords: Lymphocytes; Monocytes; Cells, Cultured; Macrophages; Humans; HIV-1; Phytohemagglutinins; DNA, Viral; Mitogens; Kinetics; Time Factors
RMID: 0030004150
DOI: 10.1006/viro.1999.0047
Appears in Collections:Microbiology and Immunology publications

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