Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/11689
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dc.contributor.authorCarr, J.-
dc.contributor.authorHocking, H.-
dc.contributor.authorLi, P.-
dc.contributor.authorBurrell, C.-
dc.date.issued1999-
dc.identifier.citationVirology, 1999; 265(2):319-329-
dc.identifier.issn0042-6822-
dc.identifier.issn2514-4138-
dc.identifier.urihttp://hdl.handle.net/2440/11689-
dc.description.abstractMacrophages are considered of central importance in cell-to-cell transmission of human immunodeficiency virus (HIV) infection in vivo. In this report, we describe a novel cell-to-cell transmission model using HIV-infected monocyte-derived macrophages (MDMs) as donor cells and peripheral blood lymphocytes (PBLs) as recipients. Virus was transmitted during a 2-h coincubation period from intracellular or tightly cell-associated viral stores in adherent infected MDMs to nonadherent CD3(+) PBLs. Transmission required cell contact, but syncytia formation was not observed. HIV cell-to-cell transmission occurred in both allogeneic and autologous systems, and replication was higher in phytohemagglutinin (PHA)-stimulated than unstimulated recipient PBLs. In contrast, transmission of infection by cell-free virus was barely detectable without PHA stimulation of recipients, suggesting the cell-cell interaction may have provided stimuli to recipient cells in the cell-to-cell system. Viral DNA levels increased 5-24 h postmixing, and this increase was inhibited by pretreatment of cells with the reverse transcription inhibitor azidothymidine, indicating de novo reverse transcription was involved. Cell-to-cell transmission was more efficient than infection with cell-free virus released from donor MDMs, or 0.1 TCID(50)/cell cell-free viral challenge. This model provides a system to further investigate the mechanisms and characteristics of HIV cell-to-cell transmission between relevant primary cells that may be analogous to this important mode of virus spread in vivo.-
dc.language.isoen-
dc.publisherACADEMIC PRESS INC-
dc.subjectLymphocytes-
dc.subjectMonocytes-
dc.subjectCells, Cultured-
dc.subjectMacrophages-
dc.subjectHumans-
dc.subjectHIV-1-
dc.subjectPhytohemagglutinins-
dc.subjectDNA, Viral-
dc.subjectMitogens-
dc.subjectKinetics-
dc.subjectTime Factors-
dc.titleRapid and efficient cell-to-cell transmission of human immunodeficiency virus infection from monocyte-derived macrophages to peripheral blood lymphocytes-
dc.typeJournal article-
dc.identifier.doi10.1006/viro.1999.0047-
pubs.publication-statusPublished-
dc.identifier.orcidBurrell, C. [0000-0002-4020-349X]-
Appears in Collections:Aurora harvest 2
Microbiology and Immunology publications

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