Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/116911
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Type: Journal article
Title: A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses
Author: Prow, N.
Liu, L.
Nakayama, E.
Cooper, T.
Yan, K.
Eldi, P.
Hazlewood, J.
Tang, B.
Le, T.
Setoh, Y.
Khromykh, A.
Hobson-Peters, J.
Diener, K.
Howley, P.
Hayball, J.
Suhrbier, A.
Citation: Nature Communications, 2018; 9(1):1230-1-1230-12
Publisher: Nature Publishing Group
Issue Date: 2018
ISSN: 2041-1723
2041-1723
Statement of
Responsibility: 
Natalie A. Prow, Liang Liu, Eri Nakayama, Tamara H. Cooper ... Kerrilyn R. Diener ... John D. Hayball .. et al.
Abstract: Zika and chikungunya viruses have caused major epidemics and are transmitted by Aedes aegypti and/or Aedes albopictus mosquitoes. The "Sementis Copenhagen Vector" (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. A single vaccination of mice induces neutralizing antibodies to both viruses in wild-type and IFNAR-/- mice and protects against (i) chikungunya virus viremia and arthritis in wild-type mice, (ii) Zika virus viremia and fetal/placental infection in female IFNAR-/- mice, and (iii) Zika virus viremia and testes infection and pathology in male IFNAR-/- mice. To our knowledge this represents the first single-vector construct, multi-pathogen vaccine encoding large polyproteins, and offers both simplified manufacturing and formulation, and reduced "shot burden" for these often co-circulating arboviruses.
Keywords: Hela Cells; CHO Cells; Vero Cells; Animals; Mice, Inbred C57BL; Humans; Cricetulus; Vaccinia virus; Chikungunya virus; Viral Vaccines; Enzyme-Linked Immunosorbent Assay; Pregnancy; Maternal-Fetal Exchange; Genetic Vectors; Female; Male; Receptor, Interferon alpha-beta; Antibodies, Neutralizing; Chikungunya Fever; Zika Virus; Zika Virus Infection; Chlorocebus aethiops
Rights: © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
RMID: 0030084347
DOI: 10.1038/s41467-018-03662-6
Grant ID: http://purl.org/au-research/grants/arc/LP160100633
Appears in Collections:Medicine publications

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