Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/117424
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dc.contributor.authorMerriman, J.A.en
dc.contributor.authorJennings, P.C.en
dc.contributor.authorMcLaughlin, E.A.en
dc.contributor.authorJones, K.T.en
dc.date.issued2012en
dc.identifier.citationBiology of Reproduction, 2012; 86(2):49-1-49-6en
dc.identifier.issn0006-3363en
dc.identifier.issn1529-7268en
dc.identifier.urihttp://hdl.handle.net/2440/117424-
dc.description.abstractHuman eggs are highly aneuploid, with female age being the only known risk factor. Here this aging phenomenon was further studied in Swiss CD1 mice aged between 1 and 15 mo. The mean number of eggs ± SEM recovered from mice following superovulation peaked at 22.5 ± 3.8 eggs/oviduct in 3-mo-old females, decreasing markedly between 6 and 9 mo old, and was only 2.1 ± 0.2 eggs/oviduct by 15 mo. Measurement of aneuploidy in these eggs revealed a low rate, ∼3-4%, in mice aged 1 and 3 mo, rising to 12.5% by 9 mo old and to 37.5% at 12 mo. Fifteen-month-old mice had the highest rate of aneuploidy, peaking at 60%. The in situ chromosome counting technique used here allowed us to measure with accuracy the distance between the kinetochores in the sister chromatids of the eggs analyzed for aneuploidy. We observed that this distance increased in eggs from older females, from 0.38 ± 0.01 μm at 1 mo old to 0.82 ± 0.03 μm by 15 mo. Furthermore, in 3- to 12-mo-old females, aneuploid eggs had significantly larger interkinetochore distances than euploid eggs from the same age, and measurements were similar to eggs from the oldest mice. However, the association between aneuploidy and interkinetochore distance was not observed at the oldest, 15-mo age, despite such measurements being maximal. We conclude that in aging CD1 mice, a reduction in the ovulated egg number precedes a rise in aneuploidy and, furthermore, except at very advanced ages, increased interkinetochore distance is associated with aneuploidy.en
dc.description.statementofresponsibilityJulie A. Merriman, Phoebe C. Jennings, Eileen A. McLaughlin, and Keith T. Jonesen
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rights© 2012 by the Society for the Study of Reproduction, Inc.en
dc.subjectAging; aneuploidy; oocyte; oocyte maturationen
dc.titleEffect of aging on superovulation efficiency, aneuploidy rates, and sister chromatid cohesion in mice aged up to 15 monthsen
dc.typeJournal articleen
dc.identifier.rmid0030095201en
dc.identifier.doi10.1095/biolreprod.111.095711en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/569202en
dc.identifier.pubid432898-
pubs.library.collectionGenetics publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidJones, K.T. [0000-0002-0294-0851]en
Appears in Collections:Genetics publications

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