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Type: Journal article
Title: Recombinant leucine-rich repeat flightless-interacting protein-1 improves healing of acute wounds through its effects on proliferation inflammation and collagen deposition
Author: Kopecki, Z.
Stevens, N.
Yang, G.
Melville, E.
Cowin, A.
Citation: International Journal of Molecular Sciences, 2018; 19(7):2014-1-2014-12
Publisher: MDPI
Issue Date: 2018
ISSN: 1661-6596
Statement of
Zlatko Kopecki, Natalie E. Stevens, Gink N. Yang, Elizabeth Melville and Allison J. Cowin
Abstract: Wound healing is an increasing clinical problem involving substantial morbidity, mortality, and rising health care costs. Leucine-rich repeat flightless-interacting protein-1 (LRRFIP-1) regulates toll-like receptor (TLR)-mediated inflammation, suggesting a potential role in the healing of wounds. We sought to determine the role of LRRFIP-1 in wound repair and whether the exogenous addition of recombinant LRRFIP-1 (rLRRFIP-1) affected healing responses. Using a model of full-thickness incisional acute wounds in BALB/c mice, we investigated the effect of wounding on LRRFIP-1 expression. The effect of rLRRFIP-1 on cellular proliferation, inflammation, and collagen deposition was also investigated. LRRFIP-1 was upregulated in response to wounding, was found to directly associate with flightless I (Flii), and significantly increased cellular proliferation both in vitro and in vivo. rLRRFIP-1 reduced Flii expression in wounds in vivo and resulted in significantly improved healing with a concurrent dampening of TLR4-mediated inflammation and improved collagen deposition. Additionally, decreased levels of TGF-β1 and increased levels of TGF-β3 were observed in rLRRFIP-1-treated wounds suggesting a possible antiscarring effect of rLRRFIP-1. Further studies are required to elucidate if the mechanisms behind LRRFIP-1 action in wound repair are independent of Flii. However, these results identify rLRRFIP-1 as a possible treatment modality for improved healing of acute wounds.
Keywords: LRRFIP-1; flightless I; wound healing; collagen
Rights: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
DOI: 10.3390/ijms19072014
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