Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/117434
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Type: | Journal article |
Title: | Recombinant leucine-rich repeat flightless-interacting protein-1 improves healing of acute wounds through its effects on proliferation inflammation and collagen deposition |
Author: | Kopecki, Z. Stevens, N.E. Yang, G.N. Melville, E. Cowin, A.J. |
Citation: | International Journal of Molecular Sciences, 2018; 19(7):2014-1-2014-12 |
Publisher: | MDPI |
Issue Date: | 2018 |
ISSN: | 1661-6596 1422-0067 |
Statement of Responsibility: | Zlatko Kopecki, Natalie E. Stevens, Gink N. Yang, Elizabeth Melville and Allison J. Cowin |
Abstract: | Wound healing is an increasing clinical problem involving substantial morbidity, mortality, and rising health care costs. Leucine-rich repeat flightless-interacting protein-1 (LRRFIP-1) regulates toll-like receptor (TLR)-mediated inflammation, suggesting a potential role in the healing of wounds. We sought to determine the role of LRRFIP-1 in wound repair and whether the exogenous addition of recombinant LRRFIP-1 (rLRRFIP-1) affected healing responses. Using a model of full-thickness incisional acute wounds in BALB/c mice, we investigated the effect of wounding on LRRFIP-1 expression. The effect of rLRRFIP-1 on cellular proliferation, inflammation, and collagen deposition was also investigated. LRRFIP-1 was upregulated in response to wounding, was found to directly associate with flightless I (Flii), and significantly increased cellular proliferation both in vitro and in vivo. rLRRFIP-1 reduced Flii expression in wounds in vivo and resulted in significantly improved healing with a concurrent dampening of TLR4-mediated inflammation and improved collagen deposition. Additionally, decreased levels of TGF-β1 and increased levels of TGF-β3 were observed in rLRRFIP-1-treated wounds suggesting a possible antiscarring effect of rLRRFIP-1. Further studies are required to elucidate if the mechanisms behind LRRFIP-1 action in wound repair are independent of Flii. However, these results identify rLRRFIP-1 as a possible treatment modality for improved healing of acute wounds. |
Keywords: | LRRFIP-1; flightless I; wound healing; collagen |
Rights: | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/ijms19072014 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1002009 |
Published version: | http://dx.doi.org/10.3390/ijms19072014 |
Appears in Collections: | Aurora harvest 8 Medicine publications |
Files in This Item:
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hdl_117434.pdf | Published version | 1.6 MB | Adobe PDF | View/Open |
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