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dc.contributor.authorMattiske, T.en
dc.contributor.authorTan, M.en
dc.contributor.authorDearsley, O.en
dc.contributor.authorCloosterman, D.en
dc.contributor.authorHii, C.en
dc.contributor.authorGécz, J.en
dc.contributor.authorShoubridge, C.en
dc.identifier.citationPLoS ONE, 2018; 13(11):1-24en
dc.description.abstractAristaless-related homeobox (ARX) gene encodes a paired-type homeodomain transcription factor with critical roles in development. Here we identify that ARX protein is phosphorylated. Using mass spectrometry and in vitro kinase assays we identify phosphorylation at serines 37, 67 and 174. Through yeast-2-hybrid and CoIP we identified PICK1 (Protein interacting with C kinase 1) binding with the C-terminal region of ARX. PICK1 is a scaffold protein known to facilitate phosphorylation of protein partners by protein kinase C alpha (PRKCA). We confirm that ARX is phosphorylated by PRKCA and demonstrate phosphorylation at serine 174. We demonstrate that phosphorylation is required for correct transcriptional activity of the ARX protein using transcriptome-wide analysis of gene expression of phospho-null mutants (alanines replacing serines) compared to ARX wild-type (ARX-WT) overexpressed in pancreatic alpha TC cells. Compared to untransfected cells, ARX-WT overexpression significantly altered expression of 70 genes (Log2FC >+/-1.0, P-value <0.05). There were fewer genes with significantly altered expression compared to untransfected cells with the double phospho-null mutant Ser37Ala+Ser67Ala (26%) and Ser174Ala (39%), respectively. We demonstrate that the c-terminal region of ARX required to bind PICK1 causes a shift in PICK1 subcellular localisation to the nucleus to co-locate with the ARX protein, and truncation of this C-terminal region leads to the same loss of transcriptional activation as S174A mutant. In conclusion, we show that ARX is phosphorylated at several sites and that this modification affects its transcriptional activity.en
dc.description.statementofresponsibilityTessa Mattiske, May H. Tan, Oliver Dearsley, Desiree Cloosterman, Charles S. Hii, Jozef Gécz, Cheryl Shoubridgeen
dc.publisherPublic Library of Scienceen
dc.rights© 2018 Mattiske et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectPhosphorylation; serine; immunoprecipitation; transcription factors; DNA transcription; gene expression; DNA-binding proteins; homeoboxen
dc.titleRegulating transcriptional activity by phosphorylation: a new mechanism for the ARX homeodomain transcription factoren
dc.typeJournal articleen
pubs.library.collectionMedicine publicationsen
dc.identifier.orcidMattiske, T. [0000-0002-9581-5370]en
dc.identifier.orcidGécz, J. [0000-0002-7884-6861]en
dc.identifier.orcidShoubridge, C. [0000-0002-0157-3084]en
Appears in Collections:Medicine publications

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