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Type: Journal article
Title: RNA catabolites contribute to the nitrogen pool and support growth recovery of wheat
Author: Melino, V.
Casartelli, A.
George, J.
Rupasinghe, T.
Roessner, U.
Okamoto, M.
Heuer, S.
Citation: Advances in Intelligent Systems and Computing, 2018; 871:1539-1-1539-20
Publisher: Frontiers Media
Issue Date: 2018
ISSN: 2194-5357
Statement of
Vanessa Jane Melino, Alberto Casartelli, Jessey George, Thusitha Rupasinghe, Ute Roessner, Mamoru Okamoto and Sigrid Heuer
Abstract: Turn-over of RNA and catabolism of nucleotides releases one to four ammonia molecules; the released nutrients being reassimilated into primary metabolism. Preliminary evidence indicates that monocots store high levels of free nucleotides and nucleosides but their potential as a source of internal organic nitrogen for use and remobilization is uncharted. Early tillering wheat plants were therefore starved of N over a 5-day time-course with examination of nucleic acid yields in whole shoots, young and old leaves and roots. Nucleic acids constituted ∼4% of the total N pool of N starved wheat plants, which was comparable with the N available from nitrate (NO3 -) and greater than that available from the sum of 20 proteinogenic amino acids. Methods were optimized to detect nucleotide (purine and pyrimidine) metabolites, and wheat orthologs of RNA degradation (TaRNS), nucleoside transport (TaENT1, TaENT3) and salvage (TaADK) were identified. It was found that N starved wheat roots actively catabolised RNA and specific purines but accumulated pyrimidines. Reduced levels of RNA corresponded with induction of TaRNS2, TaENT1, TaENT3, and TaADK in the roots. Reduced levels of GMP, guanine, xanthine, allantoin, allantoate and glyoxylate in N starved roots correlated with accumulation of allantoate and glyoxylate in the oldest leaf, suggesting translocation of allantoin. Furthermore, N starved wheat plants exogenously supplied with N in the form of purine catabolites grew and photosynthesized as well as those plants re-supplied with NO3 -. These results support the hypothesis that the nitrogen and carbon recovered from purine metabolism can support wheat growth.
Keywords: Triticum aestivum; adenosine kinase; equilibrative nucleoside transporter; nitrate; nitrogen recycling; nucleosides; purines; ribonuclease
Rights: © 2018 Melino, Casartelli, George, Rupasinghe, Roessner, Okamoto and Heuer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
RMID: 0030102981
DOI: 10.3389/fpls.2018.01539
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Appears in Collections:Agriculture, Food and Wine publications

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