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Type: Journal article
Title: Specific growth conditions induce a Streptococcus pneumoniae non-mucoidal, small colony variant and determine the outcome of its co-culture with Haemophilus influenzae
Author: Tikhomirova, A.
Trappetti, C.
Standish, A.
Zhou, Y.
Breen, J.
Pederson, S.
Zilm, P.
Paton, J.
Kidd, S.
Citation: Pathogens and Disease, 2018; 76(7)
Publisher: Oxford University Press
Issue Date: 2018
ISSN: 2049-632X
Statement of
Alexandra Tikhomirova, Claudia Trappetti, Alistair J Standish, Yiwen Zhou James Breen, Stephen Pederson, Peter S Zilm, James C Paton, Stephen P Kidd
Abstract: Haemophilus influenzae and Streptococcus pneumoniae are known aetiologic agents of chronic otitis media, frequently as a multispecies infection. In this study, we show that the outcome of H. influenzae/S. pneumoniae interactions is dependent on the nutrient source. In continuous culture containing chemically defined media with lactose, S. pneumoniae was non-viable in mono-culture, and in co-culture remained non-viable until 288 h. With glucose, S. pneumoniae became non-viable in mono-culture, but uniquely existed in 3 distinct states in co-culture: parental cells (until 24 h), a dormant state until 336 h and its re-emergence as a non-mucoidal, small colony variant (SCV). The S. pneumoniae SCV was stable and whole genome sequencing showed three major single nucleotide polymorphisms in the SCV cells-cap3A (capsule biosynthesis pathway), fpg (DNA glycosylase of the DNA repair mechanism) and glutamate-5-kinase. Previously, fpg mutants have shown increased mutator rates, permitting bacterial survival against host-generated stresses. Transcriptomics showed these SCV cells up-regulated sugar transporters and toxin/antitoxin systems. An animal model revealed a reduced survival in the lungs and ear by SCV cells. This is the first study documenting the effect of carbon source and the development of a distinct S. pneumoniae cell type during H. influenzae/S. pneumoniae interactions.
Keywords: Streptococcus pneumoniae; Haemophilus influenzae; co-infection; small colony variants; bacterial pathogenesis; molecular adaptation; dormant
Rights: © FEMS 2018. All rights reserved.
DOI: 10.1093/femspd/fty074
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Environment Institute publications

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