Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/117621
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dc.contributor.author | Kittipassorn, T. | - |
dc.contributor.author | Haydinger, C.D. | - |
dc.contributor.author | Wood, J.P.M. | - |
dc.contributor.author | Mammone, T. | - |
dc.contributor.author | Casson, R.J. | - |
dc.contributor.author | Peet, D.J. | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Experimental Eye Research, 2019; 181:127-135 | - |
dc.identifier.issn | 0014-4835 | - |
dc.identifier.issn | 1096-0007 | - |
dc.identifier.uri | http://hdl.handle.net/2440/117621 | - |
dc.description.abstract | Müller cells (MCs) play a crucial role in the retina, and cultured MC lines are an important tool with which to study MC function. Transformed MC lines have been widely used; however, the transformation process can also lead to unwanted changes compared to the primary cells from which they were derived. To provide an alternative experimental tool, a monoclonal novel spontaneously immortalized rat Müller cell line, SIRMu-1, was derived from primary rat MCs and characterized. Immunofluorescence, western blotting and RNA sequencing demonstrate that the SIRMu-1 cell line retains similar characteristics to cultured primary MCs in terms of expression of the MC markers cellular retinaldehyde-binding protein, glutamine synthetase, S100, vimentin and glial fibrillary acidic protein at both the mRNA and protein levels. Both the cellular morphology and overall transcriptome of the SIRMu-1 cells are more similar to primary rat MCs than the commonly used rMC-1 cells, a well-described, transformed rat MC line. Furthermore, SIRMu-1 cells proliferate rapidly, have an effectively indefinite life span and a high transfection efficiency. The expression of Y chromosome specific genes confirmed that the SIRMu-1 cells are derived from male MCs. Thus, the SIRMu-1 cell line represents a valuable experimental tool to study roles of MCs in both physiological and pathological states. | - |
dc.description.statementofresponsibility | Thaksaon Kittipassorn, Cameron D. Haydinger, John P.M. Wood, Teresa Mammone, Robert J. Casson, Daniel J. Peet | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | © 2019 Elsevier Ltd. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.exer.2019.01.013 | - |
dc.subject | Cell culture | - |
dc.subject | Müller cell | - |
dc.subject | Retina | - |
dc.subject | SIRMu-1 | - |
dc.subject | Spontaneously immortalized cell | - |
dc.title | Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1 | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.exer.2019.01.013 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1099932 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kittipassorn, T. [0000-0001-9854-2905] | - |
dc.identifier.orcid | Haydinger, C.D. [0000-0003-2591-0863] | - |
dc.identifier.orcid | Casson, R.J. [0000-0003-2822-4076] | - |
dc.identifier.orcid | Peet, D.J. [0000-0002-6085-8936] | - |
Appears in Collections: | Aurora harvest 8 Biochemistry publications |
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File | Description | Size | Format | |
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hdl_117621.pdf | Submitted version | 2.25 MB | Adobe PDF | View/Open |
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