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Type: Journal article
Title: Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex
Author: Sorvina, A.
Bader, C.
Darby, J.
Lock, M.
Soo, J.
Johnson, I.
Caporale, C.
Voelcker, N.
Stagni, S.
Massi, M.
Morrison, J.
Plush, S.
Brooks, D.
Citation: Scientific Reports, 2018; 8(1):8191-1-8191-8
Publisher: Springer Nature
Issue Date: 2018
ISSN: 2045-2322
Statement of
Alexandra Sorvina, Christie A. Bader, Jack R.T. Darby, Mitchell C. Lock, Jia Yin Soo, Ian R.D. Johnson, Chiara Caporale, Nicolas H. Voelcker, Stefano Stagni, Massimiliano Massi, Janna L. Morrison, Sally E. Plush, Douglas A. Brooks
Abstract: Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. However, mitochondrial imaging is currently limited by the availability of agents that have the capacity to image mitochondrial morphology in both live and fixed samples. This can be particularly problematic in clinical studies or large, multi-centre cohort studies, where tissue archiving by fixation is often more practical. We previously reported the synthesis of an iridium coordination complex [Ir(ppy)₂(MeTzPyPhCN)]+; where ppy is a cyclometalated 2-phenylpyridine and TzPyPhCN is the 5-(5-(4-cyanophen-1-yl)pyrid-2-yl)tetrazolate ligand; and showed that this complex (herein referred to as IraZolve-Mito) has a high specificity for mitochondria in live cells. Here we demonstrate that IraZolve-Mito can also effectively stain mitochondria in both live and fixed tissue samples. The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria.
Keywords: Cell Line; Mitochondria; Animals; Sheep; Rats; Iridium; Luminescent Agents; Microscopy, Confocal; Histocytological Preparation Techniques; Tissue Fixation; Cell Survival; Female; Luminescence; Coordination Complexes; Optical Imaging
Rights: © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
RMID: 0030096346
DOI: 10.1038/s41598-018-24672-w
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