Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/117688
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Type: Journal article
Title: Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia
Author: Shi, W.
Xie, Y.
He, J.
Zhou, J.
Gao, Y.
Wei, W.
Ding, N.
Ma, H.
Xian, C.
Chen, K.
Wang, J.
Citation: Scientific Reports, 2017; 7(1):1866-1-1866-12
Publisher: Springer Nature
Issue Date: 2017
ISSN: 2045-2322
2045-2322
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Responsibility: 
Wengui Shi, Yanfang Xie, Jinpeng He, Jian Zhou, Yuhai Gao, Wenjun Wei, Nan Ding, Huiping Ma, Cory J. Xian, Keming Chen, Jufang Wang
Abstract: It is well documented that microgravity in space environment leads to bone loss in astronauts. These physiological changes have also been validated by human and animal studies and modeled in cell-based analogs. However, the underlying mechanisms are elusive. In the current study, we identified a novel phenomenon that primary cilia (key sensors and functioning organelles) of rat calvarial osteoblasts (ROBs) gradually shrank and disappeared almost completely after exposure to simulated microgravity generated by a random positioning machine (RPM). Along with the abrogation of primary cilia, the differentiation, maturation and mineralization of ROBs were inhibited. We also found that the disappearance of primary cilia was prevented by treating ROBs with cytochalasin D, but not with LiCl or dynein light chain Tctex-type 1 (Dynlt1) siRNA. The repression of the differentiation, maturation and mineralization of ROBs was effectively offset by cytochalasin D treatment in microgravity conditions. Blocking ciliogenesis using intraflagellar transport protein 88 (IFT88) siRNA knockdown inhibited the ability of cytochalasin D to counteract this reduction of osteogenesis. These results indicate that the abrogation of primary cilia may be responsible for the microgravity's inhibition on osteogenesis. Reconstruction of primary cilia may become a potential strategy against bone loss induced by microgravity.
Keywords: Osteoblasts
Rights: © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
RMID: 0030102937
DOI: 10.1038/s41598-017-02049-9
Grant ID: http://purl.org/au-research/grants/nhmrc/1042105
Appears in Collections:Medicine publications

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