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Type: Journal article
Title: The late osteoblast/preosteocyte cell line MLO-A5 displays mesenchymal lineage plasticity in vitro and in vivo
Author: Yang, D.
Gronthos, S.
Isenmann, S.
Morris, H.A.
Atkins, G.J.
Citation: Stem Cells International, 2019; 2019:9838167-1-9838167-10
Publisher: Hindawi Publishing Corporation
Issue Date: 2019
ISSN: 1687-9678
Statement of
Dongqing Yang, Stan Gronthos, Sandra Isenmann Howard A. Morris and Gerald J. Atkins
Abstract: The process of osteoblast switching to alternative mesenchymal phenotypes is incompletely understood. In this study, we tested the ability of the osteoblast/preosteocyte osteogenic cell line, MLO-A5, to also differentiate into either adipocytes or chondrocytes. MLO-A5 cells expressed a subset of skeletal stem cell markers, including Sca-1, CD44, CD73, CD146, and CD166. Confluent cultures of cells underwent differentiation within 3 days upon the addition of osteogenic medium. The same cultures were capable of undergoing adipogenic and chondrogenic differentiation under lineage-appropriate culture conditions, evidenced by lineage-specific gene expression analysis by real-time reverse-transcription-PCR, and by Oil Red O and alcian blue (pH 2.5) staining, respectively. Subcutaneous implantation of MLO-A5 cells in a gel foam into NOD SCID mice resulted in a woven bone-like structure containing embedded osteocytes and regions of cartilage-like tissue, which stained positive with both alcian blue (pH 2.5) and safranin O. Together, our findings show that MLO-A5 cells, despite being a strongly osteogenic cell line, exhibit characteristics of skeletal stem cells and display mesenchymal lineage plasticity in vitro and in vivo. These unique characteristics suggest that this cell line is a useful model with which to study aging and disease-related changes to the mesenchymal lineage composition of bone.
Rights: © 2019 Dongqing Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1155/2019/9838167
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