Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/118324
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dc.contributor.author | Zhang, K. | - |
dc.contributor.author | Arman, A. | - |
dc.contributor.author | Anwer, A. | - |
dc.contributor.author | Hutchinson, M. | - |
dc.contributor.author | Goldys, E. | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 2019; 282:122-129 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/2440/118324 | - |
dc.description.abstract | Sensitive and localized measurements of cytokines is important in biomedicine as cytokines are produced locally where needed to induce an immune reaction. Here, we present a novel immunosensor deposited on the optical fiber surface. The sensor is capable of localized detection of interleukin-1beta (IL-1β) in the rat spinal cord. In this immunosensor, a stable immunocapture surface was formed via a biotin-streptavidin coupling strategy and fluorescent carboxylated supermagnetic iron oxide (SPIO)-IL-1β detection antibody conjugates were used for signal amplification. Under the optimal condition, the proposed immunosensor can be used for the estimation of IL-1β in vitro in the range from 3.13 pg.mL⁻¹ to 400 pg.mL⁻¹ with a detection limit of 1.12 pg.mL⁻¹. Furthermore, the performance of the fiber sensor was firstly assessed by ex-vivo monitoring the secretions of the rat macrophages stimulated by lipopolysaccharide (LPS), and the results demonstrated significant correlations with a commercial ELISA kit. Furthermore, the fiber sensor was successfully utilized to carry out a localized measurement of IL-1β in a spinal cord of an anesthetized rat. The result indicates that such fiber sensors can be used as an effective and sensitive tool for localized detection of IL-1β in vivo, in a range of research and clinical applications. | - |
dc.description.statementofresponsibility | Kaixin Zhang, Azim Arman, Ayad G. Anwer, Mark R. Hutchinson, Ewa M. Goldys | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | © 2018 Published by Elsevier B.V. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.snb.2018.11.054 | - |
dc.subject | Optical fiber; interleukin-1beta; in vivo; sensor; immunosensor | - |
dc.title | An optical fiber based immunosensor for localized detection of IL-1β in rat spinal cord | - |
dc.title.alternative | An optical fiber based immunosensor for localized detection of IL-1beta in rat spinal cord | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.snb.2018.11.054 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE140100003 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Hutchinson, M. [0000-0003-2154-5950] | - |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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