Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/118344
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dc.contributor.authorStaudacher, A.H.en
dc.contributor.authorLi, Y.en
dc.contributor.authorLiapis, V.en
dc.contributor.authorHou, J.J.C.en
dc.contributor.authorChin, D.en
dc.contributor.authorDolezal, O.en
dc.contributor.authorAdams, T.E.en
dc.contributor.authorvan Berkel, P.H.en
dc.contributor.authorBrown, M.P.en
dc.date.issued2019en
dc.identifier.citationMolecular Cancer Therapeutics, 2019; 18(2):335-345en
dc.identifier.issn1535-7163en
dc.identifier.issn1538-8514en
dc.identifier.urihttp://hdl.handle.net/2440/118344-
dc.description"Published first November 9, 2018."en
dc.description.abstractAntibody-drug conjugates (ADC) have revolutionized the field of cancer therapy. ADCs combine the high specificity of tumor-targeting monoclonal antibodies with potent cytotoxic drugs, which cannot be used alone because of their high toxicity. Till date, all ADCs have either targeted cell membrane proteins on tumors or the tumor vasculature and microenvironment. Here, we investigate ADCs of APOMAB (DAB4, or its chimeric derivative, chDAB4), which is a mAb targeting the La/SSB protein, which is only accessible for binding in dying or dead cancer cells. We show that DAB4-labeled dead cells are phagocytosed by macrophages, and that the apoptotic/necrotic areas within lung tumor xenografts are bound by DAB4 and are infiltrated with macrophages. We show that only DAB4-ADCs with a cleavable linker and diffusible drug are effective in two lung cancer models, particularly when given after chemotherapy. These results are consistent with other recent studies showing that direct internalization of ADCs by target cells is not essential for ADC activity because the linker can be cleaved extracellularly or through other mechanisms. Rather than targeting a tumor cell type specific antigen, DAB4-ADCs have the advantage of targeting a common trait in most solid tumors: an excess of post-apoptotic, necrotic cells either adjacent to hypoxic tumor regions or distributed more generally after cytotoxic therapy. Consequently, any antitumor effects are solely the result of bystander killing, either through internalization of the dead, ADC-bound tumor cells by macrophages, or extracellular cleavage of the ADC in the tumor microenvironment.en
dc.description.statementofresponsibilityAlexander H. Staudacher, Yanrui Li, Vasilios Liapis, Jeff Jia Cheng Hou, David Chin, Olan Dolezal, Timothy E. Adams, Patrick H. van Berkel and Michael P. Brownen
dc.language.isoenen
dc.publisherAmerican Association for Cancer Researchen
dc.rights©2018 American Association for Cancer Research.en
dc.subjectCell Line, Tumor; Macrophages; Animals; Humans; Mice; Lung Neoplasms; Antibodies, Monoclonal; Immunoconjugates; Xenograft Model Antitumor Assays; Apoptosis; Phagocytosis; Tumor Microenvironment; RAW 264.7 Cells; A549 Cellsen
dc.titleAPOMAB antibody-drug conjugates targeting dead tumor cells are effective in vivoen
dc.typeJournal articleen
dc.identifier.rmid0030102862en
dc.identifier.doi10.1158/1535-7163.MCT-18-0842en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1126304en
dc.identifier.pubid448444-
pubs.library.collectionMedicine publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidLiapis, V. [0000-0002-2354-3521]en
dc.identifier.orcidBrown, M.P. [0000-0002-5796-1932]en
Appears in Collections:Medicine publications

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