Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/118382
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Icariin attenuates methotrexate chemotherapy-induced bone marrow microvascular damage and bone loss in rats
Author: Hassanshahi, M.
Su, Y.
Khabbazi, S.
Fan, C.
Tang, Q.
Wen, X.
Fan, J.
Chen, K.
Xian, C.
Citation: Journal of Cellular Physiology, 2019; 234(9):16549-16561
Publisher: Wiley
Issue Date: 2019
ISSN: 0021-9541
1097-4652
Statement of
Responsibility: 
Mohammadhossein Hassanshahi, Yu‐Wen Su, Samira Khabbazi, Chia‐Ming Fan, Qian Tang, Xuesen Wen, Jian Fan, Ke‐Ming Chen, Cory J. Xian
Abstract: Methotrexate (MTX), a widely used antimetabolite in paediatric cancer to treatment, has been widely reported to cause bone loss and bone marrow (BM) microvascular (particularly sinusoids) damage. Investigations must now investigate how MTX-induced bone loss and microvasculature damage can be attenuated/prevented. In the present study, we examined the potency of icariin, an herbal flavonoid, in reducing bone loss and the dilation/damage of BM sinusoids in rats caused by MTX treatment. Groups of young rats were treated with five daily MTX injections (0.75 mg/kg) with and without icariin oral supplementation until Day 9 after the first MTX injection. Histological analyses showed a significant reduction in the bone volume/tissue volume (BV/TV) fraction (%) and trabecular number in the metaphysis trabecular bone of MTX-treated rats, but no significant changes in trabecular thickness and trabecular spacing. However, the BV/TV (%) and trabecular number were found to be significantly higher in MTX + icariin-treated rats than those of MTX alone-treated rats. Gene expression analyses showed that icariin treatment maintained expression of osteogenesis-related genes but suppressed the induction of adipogenesis-related genes in bones of MTX-treated rats. In addition, icariin treatment attenuated MTX-induced dilation of BM sinusoids and upregulated expression of endothelial cell marker CD31 in the metaphysis bone of icariin + MTX-treated rats. Furthermore, in vitro studies suggest that icariin treatment can potentially enhance the survival of cultured rat sinusoidal endothelial cells against cytotoxic effect of MTX and promote their migration and tube formation abilities, which is associated with enhanced production of nitric oxide.
Keywords: angiogenesis; bone loss; bone marrow sinusoids; icariin; methotrexate
Rights: © 2019 Wiley Periodicals, Inc.
RMID: 0030108894
DOI: 10.1002/jcp.28326
Grant ID: http://purl.org/au-research/grants/nhmrc/508046
http://purl.org/au-research/grants/nhmrc/508047
http://purl.org/au-research/grants/nhmrc/1042105
http://purl.org/au-research/grants/nhmrc/1127396
http://purl.org/au-research/grants/nhmrc/1010752
Appears in Collections:Medicine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.