Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/118509
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Type: Journal article
Title: Dietary butyrylated high-amylose starch reduces azoxymethane-induced colonic O⁶ -methylguanine adducts in rats as measured by immunohistochemistry and high-pressure liquid chromatography
Other Titles: Dietary butyrylated high-amylose starch reduces azoxymethane-induced colonic O(6) -methylguanine adducts in rats as measured by immunohistochemistry and high-pressure liquid chromatography
Author: Le Leu, R.
Scherer, B.
Mano, M.
Winter, J.
Lannagan, T.
Head, R.
Lockett, T.
Clarke, J.
Citation: Nutrition Research, 2016; 36(9):982-988
Publisher: Elsevier
Issue Date: 2016
ISSN: 0271-5317
1879-0739
Statement of
Responsibility: 
Richard K. Le Leu, Benjamin L. Scherer, Mark T. Mano, Jean M. Winter, Tamsin Lannagan, Richard J. Head, Trevor Lockett, Julie M. Clarke
Abstract: O⁶-methyl guanine (O⁶MeG) adducts are major toxic, promutagenic, and procarcinogenic adducts involved in colorectal carcinogenesis. Resistant starch and its colonic metabolite butyrate are known to protect against oncogenesis in the colon. In this study, we hypothesized that a dietary intervention that specifically delivers butyrate to the large bowel (notably butyrylated high-amylose maize starch [HAMSB]) would reduce colonic levels of O⁶MeG in rats shortly after exposure to the deoxyribonucleic acid (DNA) alkylating agent azoxymethane (AOM) when compared with a low-amylose maize starch (LAMS). A further objective was to validate an immunohistochemistry (IHC) method for quantifying O⁶MeG against a high-performance liquid chromatography method using fluorescence and diode array detection. Rats were fed either LAMS or HAMSB diets for 4 weeks followed by a single injection of AOM or saline and killed 6 hours later. After AOM exposure, both IHC and high-performance liquid chromatography method using fluorescence and diode array detection measured a substantially increased quantity of DNA adducts in the colon (P<.001). Both techniques demonstrated equally that consumption of HAMSB provided a protective effect by reducing colonic adduct load compared with the LAMS diet (P<.05). In addition, IHC allowed visualization of the O⁶MeG distribution, where adduct load was reduced in the lower third of the crypt compartment in HAMSB-fed rats (P=.036). The apoptotic response to AOM was higher in the HAMSB-fed rats (P=.002). In conclusion, the reduction in O(6)MeG levels and enhancement of the apoptotic response to DNA damage in the colonic epithelium through consumption of HAMSB provide mechanistic insights into how HAMSB protects against colorectal tumorigenesis.
Keywords: Resistant starch; colon; DNA adduct; butyrate; colorectal cancer; rat model
Rights: © 2016 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.nutres.2016.06.006
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