Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/118968
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Type: Journal article
Title: The role of controlled surface topography and chemistry on mouse embryonic stem cell attachment, growth and self-renewal
Author: Macgregor, M.
Williams, R.
Downes, J.
Bachhuka, A.
Vasilev, K.
Citation: Materials, 2017; 10(9):1081-1-1081-13
Publisher: MDPI
Issue Date: 2017
ISSN: 1996-1944
1996-1944
Statement of
Responsibility: 
Melanie Macgregor, Rachel Williams, Joni Downes, Akash Bachhuka and Krasimir Vasilev
Abstract: The success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation.
Keywords: plasma polymer; Polyallylamine; polyoctadiene; polyacrylic acid; nanotopography; Mouse embryonic stem cells; fibronectin; Extra Cellular Matrix physical cues; Extra Cellular Matrix chemical cues
Rights: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
RMID: 0030076124
DOI: 10.3390/ma10091081
Grant ID: http://purl.org/au-research/grants/arc/DP150104212
http://purl.org/au-research/grants/nhmrc/1122825
Appears in Collections:Chemistry and Physics publications

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