Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/118968
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dc.contributor.authorMacgregor, M.en
dc.contributor.authorWilliams, R.en
dc.contributor.authorDownes, J.en
dc.contributor.authorBachhuka, A.en
dc.contributor.authorVasilev, K.en
dc.date.issued2017en
dc.identifier.citationMaterials, 2017; 10(9):1081-1-1081-13en
dc.identifier.issn1996-1944en
dc.identifier.issn1996-1944en
dc.identifier.urihttp://hdl.handle.net/2440/118968-
dc.description.abstractThe success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation.en
dc.description.statementofresponsibilityMelanie Macgregor, Rachel Williams, Joni Downes, Akash Bachhuka and Krasimir Vasileven
dc.language.isoenen
dc.publisherMDPIen
dc.rights© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectplasma polymer; Polyallylamine; polyoctadiene; polyacrylic acid; nanotopography; Mouse embryonic stem cells; fibronectin; Extra Cellular Matrix physical cues; Extra Cellular Matrix chemical cuesen
dc.titleThe role of controlled surface topography and chemistry on mouse embryonic stem cell attachment, growth and self-renewalen
dc.typeJournal articleen
dc.identifier.rmid0030076124en
dc.identifier.doi10.3390/ma10091081en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP150104212en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1122825en
dc.identifier.pubid369977-
pubs.library.collectionChemistry and Physics publicationsen
pubs.library.teamDS06en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidBachhuka, A. [0000-0003-1253-8126]en
Appears in Collections:Chemistry and Physics publications

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