Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/119215
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Type: Journal article
Title: Manipulating the mitochondrial genome to enhance cattle embryo development
Author: Srirattana, K.
St John, J.C.
Citation: G3 : Genes, Genomes, Genetics, 2017; 7(7):2065-2080
Publisher: Genetics Society of America
Issue Date: 2017
ISSN: 2160-1836
2160-1836
Statement of
Responsibility: 
Kanokwan Srirattana and Justin C. St. John
Abstract: The mixing of mitochondrial DNA (mtDNA) from the donor cell and the recipient oocyte in embryos and offspring derived from somatic cell nuclear transfer (SCNT) compromises genetic integrity and affects embryo development. We set out to generate SCNT embryos that inherited their mtDNA from the recipient oocyte only, as is the case following natural conception. While SCNT blastocysts produced from Holstein (Bos taurus) fibroblasts depleted of their mtDNA, and oocytes derived from Angus (Bos taurus) cattle possessed oocyte mtDNA only, the coexistence of donor cell and oocyte mtDNA resulted in blastocysts derived from nondepleted cells. Moreover, the use of the reprogramming agent, Trichostatin A (TSA), further improved the development of embryos derived from depleted cells. RNA-seq analysis highlighted 35 differentially expressed genes from the comparison between blastocysts generated from nondepleted cells and blastocysts from depleted cells, both in the presence of TSA. The only differences between these two sets of embryos were the presence of donor cell mtDNA, and a significantly higher mtDNA copy number for embryos derived from nondepleted cells. Furthermore, the use of TSA on embryos derived from depleted cells positively modulated the expression of CLDN8, TMEM38A, and FREM1, which affect embryonic development. In conclusion, SCNT embryos produced by mtDNA depleted donor cells have the same potential to develop to the blastocyst stage without the presumed damaging effect resulting from the mixture of donor and recipient mtDNA.
Keywords: Mitochondrial DNA; depletion; cattle; somatic cell nuclear transfer; embryo development
Rights: Copyright © 2017 Srirattana and St. John This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030112257
DOI: 10.1534/g3.117.042655
Appears in Collections:Medicine publications

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