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Type: Journal article
Title: Hypoxia increases persistent sodium current in rat ventricular myocytes
Author: Ju, Y.
Saint, D.
Gage, P.
Citation: The Journal of Physiology, 1996; 497(2):337-347
Publisher: The Physiological Society
Issue Date: 1996
ISSN: 0022-3751
Statement of
Y.-K. Ju, D. A. Saint, and P. W. Gage
Abstract: 1. A persistent inward current activated by depolarization was recorded using the whole-cell, tight seal technique in rat isolated cardiac myocytes. The amplitude of the inward current increased when cells were exposed to a solution with low oxygen tension. 2. The persistent inward current had the characteristics of the persistent Na+ current described previously in rat ventricular myocytes: it was activated at negative potentials (-70 mV), reversed close to the equilibrium potential for Na+ (ENa), was blocked by TTX and was resistant to inactivation. 3. Persistent single Na+ channel currents activated by long (200-400 ms) depolarizations were recorded in cell-attached patches on isolated ventricular myocytes. Hypoxia increased the frequency of opening of the persistent Na+ channels. 4. Persistent Na+ channels recorded during hypoxia had characteristics similar to those of persistent Na+ channels recorded at normal oxygen tensions. They had a null potential at ENa, their amplitude varied with [Na+], they were resistant to inactivation and their mean open time increased with increasing depolarization. 5. The persistent Na+ channels in cell-attached patches were blocked by TTX (50 microM) in the patch pipette and by lidocaine (100 microM). 6. It was concluded that hypoxia increases the open probability of TTX-sensitive, inactivation-resistant Na+ channels. The voltage dependence of these channels, and their greatly increased activity during hypoxia, suggest that they may play an important role in the generation of arrhythmias during hypoxia.
Keywords: Myocardium
Heart Ventricles
Rats, Wistar
Sodium Channels
Anti-Arrhythmia Agents
Ion Channel Gating
Membrane Potentials
Time Factors
DOI: 10.1113/jphysiol.1996.sp021772
Published version:
Appears in Collections:Aurora harvest 2
Physiology publications

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