Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/120020
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Type: Journal article
Title: Development of a polyclonal anti-dugong immunoglobulin G (IgG) antibody with evaluation of total plasma IgG in a living dugong (Dugong dugon) population
Author: Wong, A.
Lanyon, J.
McKee, S.
Linedale, R.
Woolford, L.
Long, T.
Leggatt, G.
Citation: Veterinary Immunology and Immunopathology, 2018; 200:16-25
Publisher: Elsevier
Issue Date: 2018
ISSN: 0165-2427
1873-2534
Statement of
Responsibility: 
Arthur Wong, Janet M. Lanyon, Sara J. McKee, Richard Linedale, Lucy Woolford, Trevor Long, Graham R. Leggatt
Abstract: Species-specific antibodies (Ab) for the measurement of immunoglobulins (Ig) are valuable tools for determining the humoral immune status of threatened and endangered wildlife species such as dugongs. However, no studies have reported antibody reagents against dugong immunoglobulin. The object of this study was to develop an Ab with specificity for dugong IgG and apply this tool to survey total IgG levels in plasma samples from a live wild population of dugongs in southern Queensland, Australia. Dugong IgG was isolated from plasma by protein A/G column chromatography and a polyclonal antiserum was successfully raised against the dugong IgG through immunization of mice. The anti-dugong antiserum was reactive with dugong serum but not immunoglobulin from other species such as rats and humans. When tested against a panel of dugong plasma samples, relative IgG levels from dugongs (n = 116) showed biologically relevant relationships with pregnancy status and a principal component of Body Mass Index (BMI)/globulin/fecal glucocorticosteroid (chronic stress) levels combined, which together accounted for 9.2% of the variation in total Ig levels. Together these data suggest that dugongs show variation in total IgG and that this correlates with some physiological parameters of dugong health.
Keywords: Immunoglobulin; dugong; antibody
Rights: © 2018 Elsevier B.V. All rights reserved.
RMID: 0030086604
DOI: 10.1016/j.vetimm.2018.04.003
Appears in Collections:Animal and Veterinary Sciences publications

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