Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/120525
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Type: Journal article
Title: Surface modification by allylamine plasma polymerization promotes osteogenic differentiation of human adipose-derived stem cells
Author: Liu, X.
Feng, Q.
Bachhuka, A.
Vasilev, K.
Citation: ACS Applied Materials and Interfaces, 2014; 6(12):9733-9741
Publisher: ACS Publications
Issue Date: 2014
ISSN: 1944-8244
1944-8252
Statement of
Responsibility: 
Xujie Liu, Qingling Feng, Akash Bachhuka and Krasimir Vasilev
Abstract: Tuning the material properties in order to control the cellular behavior is an important issue in tissue engineering. It is now well-established that the surface chemistry can affect cell adhesion, proliferation, and differentiation. In this study, plasma polymerization, which is an appealing method for surface modification, was employed to generate surfaces with different chemical compositions. Allylamine (AAm), acrylic acid (AAc), 1,7-octadiene (OD), and ethanol (ET) were used as precursors for plasma polymerization in order to generate thin films rich in amine (-NH2), carboxyl (-COOH), methyl (-CH3), and hydroxyl (-OH) functional groups, respectively. The surface chemistry was characterized by X-ray photoelectron spectroscopy (XPS), the wettability was determined by measuring the water contact angles (WCA) and the surface topography was imaged by atomic force microscopy (AFM). The effects of surface chemical compositions on the behavior of human adipose-derive stem cells (hASCs) were evaluated in vitro: Cell Count Kit-8 (CCK-8) analysis for cell proliferation, F-actin staining for cell morphology, alkaline phosphatase (ALP) activity analysis, and Alizarin Red S staining for osteogenic differentiation. The results show that AAm-based plasma-polymerized coatings can promote the attachment, spreading, and, in turn, proliferation of hASCs, as well as promote the osteogenic differentiation of hASCs, suggesting that plasma polymerization is an appealing method for the surface modification of scaffolds used in bone tissue engineering.
Keywords: Plasma polymerization; surface modification; osteogenic differentiation; human adipose-derived stem cell; bone tissue engineering
Rights: © 2014 American Chemical Society
RMID: 0030068731
DOI: 10.1021/am502170s
Grant ID: http://purl.org/au-research/grants/arc/FT100100292
Appears in Collections:Chemistry and Physics publications
Chemistry publications

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