Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/120561
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Type: Journal article
Title: The influence of substrate stiffness gradients on primary human dermal fibroblasts
Author: Hopp, I.
Michelmore, A.
Smith, L.
Robinson, D.
Bachhuka, A.
Mierczynska, A.
Vasilev, K.
Citation: Biomaterials, 2013; 34(21):5070-5077
Publisher: Elsevier
Issue Date: 2013
ISSN: 0142-9612
1878-5905
Statement of
Responsibility: 
Isabel Hopp, Andrew Michelmore, Louise E. Smith, David E. Robinson, Akash Bachhuka, Agnieszka Mierczynska, Krasimir Vasilev
Abstract: Materials mechanical properties are known to be an important regulator of cellular processes such as proliferation, differentiation and migration, and have seen increasing attention in recent years. At present, there are only few approaches where the mechanical properties of thin films can be controllably varied across an entire surface. In this work, we present a technique for controlled generation of gradients of surface elastic moduli involving a weak polyelectrolyte multilayer (PEM) system of approximately 100 nm thickness and time dependent immersion in a solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as a crosslinking agent. Uniform surface chemistry across the gradient and wettability was provided by the addition of a 10 nm thick plasma polymer layer deposited from vapour of either allylamine or acrylic acid. We used the resultant stiffness gradients (0.5-110 MPa in hydrated state) to investigate the adhesion, morphology and proliferation on human dermal fibroblasts (HDFs). We show that substrate mechanical properties strongly influence HDF cell fate. We also found that in the experimental range of surface properties used in this study, the surface stiffness was a stronger driving force to cells fate compared to chemistry and wettability.
Keywords: Surface stiffness; young modulus; fibroblasts; cell adhesion and proliferation; layer-by-layer
Rights: © 2013 Elsevier Ltd. All rights reserved.
RMID: 0030068732
DOI: 10.1016/j.biomaterials.2013.03.075
Grant ID: http://purl.org/au-research/grants/arc/FT100100292
Appears in Collections:Chemistry and Physics publications

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