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Type: Journal article
Title: Maximizing RNA loading for gene silencing using porous silicon nanoparticles
Author: Tieu, T.
Dhawan, S.
Haridas, V.
Butler, L.
Thissen, H.
Cifuentes-Rius, A.
Voelcker, N.
Citation: ACS Applied Materials and Interfaces, 2019; 11(26):22993-23005
Publisher: American Chemical Society
Issue Date: 2019
ISSN: 1944-8244
Statement of
Terence Tieu, Sameer Dhawan, V. Haridas, Lisa M. Butler, Helmut Thissen, Anna Cifuentes-Rius, Nicolas H. Voelcker
Abstract: Gene silencing by RNA interference is a powerful technology with broad applications. However, this technology has been hampered by the instability of small interfering RNA (siRNA) molecules in physiological conditions and their inefficient delivery into the cytoplasm of target cells. Porous silicon nanoparticles have emerged as a potential delivery vehicle to overcome these limitations-being able to encapsulate RNA molecules within the porous matrix and protect them from degradation. Here, key variables were investigated that influence siRNA loading into porous silicon nanoparticles. The effect of modifying the surface of porous silicon nanoparticles with various amino-functional molecules as well as the effects of salt and chaotropic agents in facilitating siRNA loading was examined. Maximum siRNA loading of 413 μg/(mg of porous silicon nanoparticles) was found when the nanoparticles were modified by a fourth generation polyamidoamine dendrimer. Low concentrations of urea or salt increased loading capacity: an increase in RNA loading by 19% at a concentration of 0.05 M NaCl or 21% at a concentration of 0.25 M urea was observed when compared to loading in water. Lastly, it was demonstrated that dendrimer-functionalized nanocarriers are able to deliver siRNA against ELOVL5, a target for the treatment of advanced prostate cancer.
Keywords: RNA; gene delivery; nanoparticles; porous silicon; siRNA
Rights: © 2019 American Chemical Society
RMID: 0030119489
DOI: 10.1021/acsami.9b05577
Grant ID:
Appears in Collections:Chemical Engineering publications

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