Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: A 3-D in vitro co-culture model of mammary gland involution
Author: Campbell, J.
Botos, L.
Sargeant, T.
Davidenko, N.
Cameron, R.
Watson, C.
Citation: Integrative Biology: interdisciplinary approaches for molecular and cellular life sciences, 2014; 6(6):618-626
Publisher: Royal Society of Chemistry
Issue Date: 2014
ISSN: 1757-9694
Statement of
Jonathan J. Campbell, Laur-Alexandru Botos, Timothy J. Sargeant, Natalia Davidenko, Ruth E. Cameron, Christine J. Watson
Abstract: Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring. These processes are mediated in part through Jak/Stat signalling pathways, which can be deregulated in breast cancer. Synthetic in vitro analogues of the breast could become important tools for studying tumorigenic processes, or as personalized drug discovery platforms and predictors of therapeutic response. Ideally, such models should support 3D neo-tissue formation, so as to recapitulate physiological organ function, and be compatible with high-throughput screening methodologies. We have combined cell lines of epithelial, stromal and immunological origin within engineered porous collagen/ hyaluronic acid matrices, demonstrating 3D-specific molecular signatures. Furthermore seeded cells form mammary-like branched tissues, with lobuloalveolar structures that undergo inducible involution phenotypes reminiscent of the native gland under hormonal/cytokine regulation. We confirm that autophagy is mediated within differentiated mammary epithelial cells in a Stat-dependent manner at early time points following the removal of a prolactin stimulus (H/WD). In addition, epithelial cells express markers of an M2 macrophage lineage under H/WD, a process that is attenuated with the introduction of the monocyte/macrophage cell line RAW 264.7. Thus, such 3D models are suitable platforms for studying cell–cell interactions and cell death mechanisms in relation to cancer.
Keywords: 3T3-L1 Cells
Rights: This journal is © The Royal Society of Chemistry 2014
DOI: 10.1039/c3ib40257f
Appears in Collections:Aurora harvest 8
Medicine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.