Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/120779
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dc.contributor.authorVanlancker, E.en
dc.contributor.authorVanhoecke, B.en
dc.contributor.authorStringer, A.en
dc.contributor.authorVan de Wiele, T.en
dc.date.issued2017en
dc.identifier.citationPeerJ, 2017; 2017(11):e4017-1-e4017-20en
dc.identifier.issn2167-8359en
dc.identifier.issn2167-8359en
dc.identifier.urihttp://hdl.handle.net/2440/120779-
dc.description.abstractGastrointestinal mucositis is a debilitating side effect of chemotherapy treatment, with currently no treatment available. As changes in microbial composition have been reported upon chemotherapy treatment in vivo, it is thought that gut microbiota dysbiosis contribute to the mucositis etiology. Yet it is not known whether chemotherapeutics directly cause microbial dysbiosis, thereby increasing mucositis risk, or whether the chemotherapeutic subjected host environment disturbs the microbiome thereby aggravating the disease. To address this question, we used the M-SHIME®, an in vitro mucosal simulator of the human intestinal microbial ecosystem, as an experimental setup that excludes the host factor. The direct impact of two chemotherapeutics, 5-fluorouracil (5-FU) and SN-38 (active metabolite of irinotecan), on the luminal and mucosal gut microbiota from several human donors was investigated through monitoring fermentation activity and next generation sequencing. At a dose of 10 µM in the mucosal environment, 5-FU impacted the functionality and composition of the colon microbiota to a minor extent. Similarly, a daily dose of 10 µM SN-38 in the luminal environment did not cause significant changes in the functionality or microbiome composition. As our mucosal model does not include a host-compartment, our findings strongly indicate that a putative microbial contribution to mucositis is initially triggered by an altered host environment upon chemotherapy.en
dc.description.statementofresponsibilityEline Vanlancker, Barbara Vanhoecke, Andrea Stringer and Tom Van de Wieleen
dc.language.isoenen
dc.publisherPEER Jen
dc.rights© 2017 Vanlancker et al. Distributed under Creative Commons CC-BY 4.0.en
dc.subject16S rRNA sequencing; 5-Fluorouracil; Bacteria; Chemotherapy; Colon microbiota; Community analysis; Gut; Illumina amplicon sequencing; Irinotecan; Short chain fatty acidsen
dc.title5-Fluorouracil and irinotecan (SN-38) have limited impact on colon microbial functionality and composition in vitroen
dc.typeJournal articleen
dc.identifier.rmid0030132393en
dc.identifier.doi10.7717/peerj.4017en
dc.identifier.pubid387985-
pubs.library.collectionMedicine publicationsen
pubs.library.teamDS10en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidStringer, A. [0000-0003-3245-5360]en
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