Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/120983
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Type: Journal article
Title: Novel noninvasive assessment of microvascular structure and function in humans
Author: Smith, K.J.
Argarini, R.
Carter, H.H.
Quirk, B.C.
Haynes, A.
Naylor, L.H.
McKirdy, H.
Kirk, R.W.
McLaughlin, R.A.
Green, D.J.
Citation: Medicine and Science in Sports and Exercise, 2019; 51(7):1558-1565
Publisher: Wolters Kluwer
Issue Date: 2019
ISSN: 0195-9131
1530-0315
Statement of
Responsibility: 
Kurt J. Smith, Raden Argarini, Howard H. Carter, Bryden C. Quirk, Andrew Haynes, Louise H. Naylor, Hamish McKirdy, Rodney W. Kirk, Robert A. McLaughlin, and Daniel J. Green
Abstract: Introduction: Optical coherence tomography (OCT) is a novel high-resolution imaging technique capable of visualizing in vivo structures at a resolution of ~10 μm. We have developed specialized OCT-based approaches that quantify diameter, speed, and flow rate in human cutaneous microvessels. In this study, we hypothesized that OCT-based microvascular assessments would possess comparable levels of reliability when compared with those derived using conventional laser Doppler flowmetry (LDF). Methods: Speckle decorrelation images (OCT) and red blood cell flux (LDF) measures were collected from adjacent forearm skin locations on 2 d (48 h apart), at baseline, and after a 30-min rapid local heating protocol (30°C-44°C) in eight healthy young individuals. OCT postprocessing quantified cutaneous microvascular diameter, speed, flow rate, and density (vessel recruitment) within a region of interest, and data were compared between days. Results: Forearm skin LDF (13 ± 4 to 182 ± 31 AU, P < 0.05) and OCT-derived diameter (41.8 ± 6.6 vs 64.5 ± 6.9 μm), speed (68.4 ± 9.5 vs 89.0 ± 7.3 μm·s), flow rate (145.0 ± 60.6 vs 485 ± 132 pL·s), and density (9.9% ± 4.9% vs 45.4% ± 5.9%) increased in response to local heating. The average OCT-derived microvascular flow response (pL·s) to heating (234% increase) was lower (P < 0.05) than the LDF-derived change (AU) (1360% increase). Pearson correlation was significant for between-day local heating responses in terms of OCT flow (r = 0.93, P < 0.01), but not LDF (P = 0.49). Bland-Altman analysis revealed that between-day baseline OCT-derived flow rates were less variable than LDF-derived flux. Conclusions: Our findings indicate that OCT, which directly visualizes human microvessels, not only allows microvascular quantification of diameter, speed, flow rate, and vessel recruitment but also provides outputs that are highly reproducible. OCT is a promising novel approach that enables a comprehensive assessment of cutaneous microvascular structure and function in humans.
Keywords: Skin; microvascular; optical imaging; laser Doppler; cutaneous
Rights: © 2019 by the American College of Sports Medicine
RMID: 0030107605
DOI: 10.1249/MSS.0000000000001898
Grant ID: http://purl.org/au-research/grants/arc/DP160104175
http://purl.org/au-research/grants/arc/CE140100003
Appears in Collections:Medicine publications

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