Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/121049
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Type: | Journal article |
Title: | Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68 |
Author: | Truong, J.Q. Panjikar, S. Shearwin-Whyatt, L. Bruning, J.B. Shearwin, K.E. |
Citation: | Acta Crystallographica Section D: Structural Biology, 2019; 75(7):670-681 |
Publisher: | Wiley |
Issue Date: | 2019 |
ISSN: | 2059-7983 2059-7983 |
Statement of Responsibility: | Jia Quyen Truong, Santosh Panjikar, Linda Shearwin-Whyatt, John B. Bruning and Keith E. Shearwin |
Abstract: | Two commonly encountered bottlenecks in the structure determination of a protein by X-ray crystallography are screening for conditions that give high-quality crystals and, in the case of novel structures, finding derivatization conditions for experimental phasing. In this study, the phasing molecule 5-amino-2,4,6-triiodoisophthalic acid (I3C) was added to a random microseed matrix screen to generate high-quality crystals derivatized with I3C in a single optimization experiment. I3C, often referred to as the magic triangle, contains an aromatic ring scaffold with three bound I atoms. This approach was applied to efficiently phase the structures of hen egg-white lysozyme and the N-terminal domain of the Orf11 protein from Staphylococcus phage P68 (Orf11 NTD) using SAD phasing. The structure of Orf11 NTD suggests that it may play a role as a virion-associated lysin or endolysin. |
Keywords: | I3C crystallography lysin magic triangle phasing random microseed matrix screening seeding |
Rights: | © The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
DOI: | 10.1107/s2059798319009008 |
Grant ID: | http://purl.org/au-research/grants/arc/DP160101450 http://purl.org/au-research/grants/arc/DP150103009 |
Published version: | http://dx.doi.org/10.1107/s2059798319009008 |
Appears in Collections: | Aurora harvest 4 Biochemistry publications |
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hdl_121049.pdf | Published Version | 2.94 MB | Adobe PDF | View/Open |
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