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Type: Journal article
Title: Encapsulation, visualization and expression of genes with biomimetically mineralized zeolitic Imidazolate framework-8 (ZIF-8)
Author: Poddar, A.
Conesa, J.J.
Liang, K.
Dhakal, S.
Reineck, P.
Bryant, G.
Pereiro, E.
Ricco, R.
Amenitsch, H.
Doonan, C.
Mulet, X.
Doherty, C.M.
Falcaro, P.
Shukla, R.
Citation: Small, 2019; 15(36):1902268-1-1902268-7
Publisher: Wiley
Issue Date: 2019
ISSN: 1613-6810
Statement of
Arpita Poddar, José J. Conesa, Kang Liang, Sudip Dhakal ... Christian Doonan ... Paolo Falcaro ... et al.
Abstract: Recent work in biomolecule-metal-organic framework (MOF) composites has proven to be an effective strategy for the protection of proteins. However, for other biomacromolecules such as nucleic acids, the encapsulation into nano MOFs and the related characterizations are in their infancy. Herein, encapsulation of a complete gene-set in zeolitic imidazolate framework-8 (ZIF-8) MOFs and cellular expression of the gene delivered by the nano MOF composites are reported. Using a green fluorescent protein (GFP) plasmid (plGFP) as a proof-of-concept genetic macromolecule, successful transfection of mammalian cancer cells with plGFP for up to 4 days is shown. Cell transfection assays and soft X-ray cryo-tomography (cryo-SXT) demonstrate the feasibility of DNA@MOF biocomposites as intracellular gene delivery vehicles. Expression occurs over relatively prolonged time points where the cargo nucleic acid is released gradually in order to maintain sustained expression.
Keywords: DNA function
gene therapy
Rights: © 2019 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI: 10.1002/smll.201902268
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Physics publications

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