Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/121082
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dc.contributor.authorIrani, Y.-
dc.contributor.authorScotney, P.-
dc.contributor.authorNash, A.-
dc.contributor.authorWilliams, K.-
dc.date.issued2016-
dc.identifier.citationInvestigative Ophthalmology and Visual Science, 2016; 57(2):586-591-
dc.identifier.issn0146-0404-
dc.identifier.issn1552-5783-
dc.identifier.urihttp://hdl.handle.net/2440/121082-
dc.description.abstractPurpose: The species cross-reactivity of the monoclonal antibodies infliximab, bevacizumab, and an anti-VEGF-B antibody, 2H10, in humans and rodents was determined. Methods: The binding of infliximab to human, mouse, and rat TNF-α, of bevacizumab to human, mouse, and rat VEGF-A, and of the 2H10 antibody to human, mouse, and rat VEGF-B was evaluated by ELISA. The sequence of human, mouse, and rat TNF-α and VEGF-A at the binding sites for infliximab and bevacizumab were compared. Results: Infliximab bound to human TNF-α, but no binding to mouse or rat TNF-α was detected between 10 pg/mL and 10 μg/ml. Sequence comparison of the binding site revealed four changes in mouse and five in rat TNF-α compared with human. Bevacizumab bound strongly to human VEGF-A, but showed 5-log weaker binding to both mouse and rat VEGF-A. There was a single amino acid substitution in mouse and rat VEGF-A at the bevacizumab binding site. The 2H10 antibody displayed a similar binding profile to human, mouse, and rat VEGF-B. Conclusions: The species cross-reactivity of monoclonal antibodies should be determined prior to their use in preclinical animal models. The 2H10 antibody binds to human, mouse, and rat VEGF-B making it suitable for testing in rodent models of human disease.-
dc.description.statementofresponsibilityYazad Irani, Pierre Scotney, Andrew Nash, and Keryn A. Williams-
dc.language.isoen-
dc.publisherAssociation for Research in Vision and Ophthalmology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectBiologic; species cross-reactivity; monoclonal antibody; TNF-α; VEGF-A; VEGF-B; human; mouse; rat-
dc.titleSpecies cross-reactivity of antibodies used to treat ophthalmic conditions-
dc.typeJournal article-
dc.identifier.doi10.1167/iovs.15-18239-
dc.relation.grantNHMRC-
pubs.publication-statusPublished-
dc.identifier.orcidIrani, Y. [0000-0002-2861-5774]-
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Opthalmology & Visual Sciences publications

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