Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/121082
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Full metadata record
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dc.contributor.author | Irani, Y. | - |
dc.contributor.author | Scotney, P. | - |
dc.contributor.author | Nash, A. | - |
dc.contributor.author | Williams, K.A. | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Investigative Ophthalmology and Visual Science, 2016; 57(2):586-591 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.issn | 1552-5783 | - |
dc.identifier.uri | http://hdl.handle.net/2440/121082 | - |
dc.description.abstract | Purpose: The species cross-reactivity of the monoclonal antibodies infliximab, bevacizumab, and an anti-VEGF-B antibody, 2H10, in humans and rodents was determined. Methods: The binding of infliximab to human, mouse, and rat TNF-α, of bevacizumab to human, mouse, and rat VEGF-A, and of the 2H10 antibody to human, mouse, and rat VEGF-B was evaluated by ELISA. The sequence of human, mouse, and rat TNF-α and VEGF-A at the binding sites for infliximab and bevacizumab were compared. Results: Infliximab bound to human TNF-α, but no binding to mouse or rat TNF-α was detected between 10 pg/mL and 10 μg/ml. Sequence comparison of the binding site revealed four changes in mouse and five in rat TNF-α compared with human. Bevacizumab bound strongly to human VEGF-A, but showed 5-log weaker binding to both mouse and rat VEGF-A. There was a single amino acid substitution in mouse and rat VEGF-A at the bevacizumab binding site. The 2H10 antibody displayed a similar binding profile to human, mouse, and rat VEGF-B. Conclusions: The species cross-reactivity of monoclonal antibodies should be determined prior to their use in preclinical animal models. The 2H10 antibody binds to human, mouse, and rat VEGF-B making it suitable for testing in rodent models of human disease. | - |
dc.description.statementofresponsibility | Yazad Irani, Pierre Scotney, Andrew Nash, and Keryn A. Williams | - |
dc.language.iso | en | - |
dc.publisher | Association for Research in Vision and Ophthalmology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.source.uri | http://dx.doi.org/10.1167/iovs.15-18239 | - |
dc.subject | Biologic; species cross-reactivity; monoclonal antibody; TNF-α; VEGF-A; VEGF-B; human; mouse; rat | - |
dc.title | Species cross-reactivity of antibodies used to treat ophthalmic conditions | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1167/iovs.15-18239 | - |
dc.relation.grant | NHMRC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Irani, Y. [0000-0002-2861-5774] | - |
Appears in Collections: | Aurora harvest 8 Opthalmology & Visual Sciences publications |
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hdl_121082.pdf | Published version | 405.46 kB | Adobe PDF | View/Open |
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