Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/121505
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Type: Journal article
Title: Role of dynamin in elongated cell migration in a 3D matrix
Author: Lees, J.G.
Gorgani, N.N.
Ammit, A.J.
McCluskey, A.
Robinson, P.J.
O'Neill, G.M.
Citation: BBA: Molecular Cell Research, 2015; 1853(3):611-618
Publisher: Elsevier
Issue Date: 2015
ISSN: 0167-4889
1879-2596
Statement of
Responsibility: 
Justin G. Lees, Nick N. Gorgani, Alaina J. Ammit, Adam McCluskey, Phillip J. Robinson, Geraldine M. O'Neill
Abstract: The use of 3-dimensional (3D) collagen gels has yielded new insights into the migratory behaviour of cancer cells. While the large GTPase dynamin has emerged as an important regulator of cancer cell migration and invasion under 2D conditions, its role in 3D migration is unclear. We have used a potent dynamin modulator, a bis-tyrphostin derivative, Ryngo® 1-23, to investigate the role of dynamin in 3D migration in 3 different cell lines. The compound specifically inhibits persistent, elongated 3D migration in U87MG and SMA-560 cells. Treated U87MG cells adopt a rounded morphology that is not due to apoptosis, loss of matrix metalloprotease activity or inhibition of clathrin-mediated endocytosis. Given that Ryngo 1-23 is known to regulate dynamin oligomerisation and actin dynamics at the leading edge, we analysed actin filament distribution. Ryngo 1-23 induced a switch in actin filament organization in 3D cultures resulting in the generation of multiple short actin-rich microspikes. Correlated with the change in actin filament distribution, cells displayed reduced collagen gel contraction. Since acto-myosin force transmission to the extra-cellular matrix underpins persistent, elongated migration, our results suggest that Ryngo 1-23 modulates this process in 3D migration via dynamin-mediated regulation of acto-myosin force transmission to the extra-cellular matrix.
Keywords: Cell migration; 3D matrix; dynamin; cancer biology; invasion
Rights: Crown Copyright © 2014 Published by Elsevier B.V. All rights reserved.
DOI: 10.1016/j.bbamcr.2014.12.008
Grant ID: http://purl.org/au-research/grants/nhmrc/512251
http://purl.org/au-research/grants/nhmrc/632515
http://purl.org/au-research/grants/nhmrc/1032771
Published version: http://dx.doi.org/10.1016/j.bbamcr.2014.12.008
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