Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

Zhao, K.; Bleackley, M.; Chisanga, D.; Gangoda, L.; Fonseka, P.; Liem, M.; Kalra, H.; Al Saffar, H.; Keerthikumar, S.; Ang, C.-S.; Adda, C.G.; Jiang, L.; Yap, K.; Poon, I.K.; Lock, P.; Bulone, V.; Anderson, M.A.; Mathivanan, S.

Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/121541

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Type:	Journal article
Title:	Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
Author:	Zhao, K. Bleackley, M. Chisanga, D. Gangoda, L. Fonseka, P. Liem, M. Kalra, H. Al Saffar, H. Keerthikumar, S. Ang, C.-S. Adda, C.G. Jiang, L. Yap, K. Poon, I.K. Lock, P. Bulone, V. Anderson, M.A. Mathivanan, S.
Citation:	Communications Biology, 2019; 2(1):1-13
Publisher:	Springer Nature
Issue Date:	2019
ISSN:	2399-3642 2399-3642
Statement of Responsibility:	Kening Zhao, Mark Bleackley, David Chisanga, Lahiru Gangoda, Pamali Fonseka, Michael Liem, Hina Kalra, Haidar Al Saffar, Shivakumar Keerthikumar, Ching-Seng Ang, Christopher G. Adda, Lanzhou Jiang, Kuok Yap, Ivan K. Poon, Peter Lock, Vincent Bulone, Marilyn Anderson, Suresh Mathivanan
Abstract:	Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.
Keywords:	Cell Wall Saccharomyces cerevisiae Antifungal Agents Proteomics Cell Survival Mutation Stress, Physiological Endosomal Sorting Complexes Required for Transport Extracellular Vesicles Caspofungin
Rights:	© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DOI:	10.1038/s42003-019-0538-8
Grant ID:	http://purl.org/au-research/grants/arc/DP160100309 http://purl.org/au-research/grants/arc/DE150101777 http://purl.org/au-research/grants/arc/DP170102312 http://purl.org/au-research/grants/arc/FT180100333
Published version:	http://dx.doi.org/10.1038/s42003-019-0538-8
Appears in Collections:	Agriculture, Food and Wine publications Aurora harvest 8

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