Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/121639
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Type: Journal article
Title: Manipulating human dendritic cell phenotype and function with targeted porous silicon nanoparticles
Author: Stead, S.
McInnes, S.
Kireta, S.
Rose, P.
Jesudason, S.
Rojas-Canales, D.
Warther, D.
Cunin, F.
Durand, J.
Drogemuller, C.
Carroll, R.
Coates, P.
Voelcker, N.
Citation: Biomaterials, 2018; 155:92-102
Publisher: Elsevier
Issue Date: 2018
ISSN: 0142-9612
1878-5905
Statement of
Responsibility: 
Sebastian O. Stead, Steven J.P. McInnes, Svjetlana Kireta, Peter D. Rose, Shilpanjali Jesudason, Darling Rojas-Canales, David Warther, Frédérique Cunin, Jean-Olivier Durand, Christopher J. Drogemuller, Robert P. Carroll, P. Toby Coates, Nicolas H. Voelcker
Abstract: Dendritic cells (DC) are the most potent antigen-presenting cells and are fundamental for the establishment of transplant tolerance. The Dendritic Cell-Specific Intracellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN; CD209) receptor provides a target for dendritic cell therapy. Biodegradable and high-surface area porous silicon (pSi) nanoparticles displaying anti-DC-SIGN antibodies and loaded with the immunosuppressant rapamycin (Sirolimus) serve as a fit-for-purpose platform to target and modify DC. Here, we describe the fabrication of rapamycin-loaded DC-SIGN displaying pSi nanoparticles, the uptake efficiency into DC and the extent of nanoparticle-induced modulation of phenotype and function. DC-SIGN antibody displaying pSi nanoparticles favourably targeted and were phagocytosed by monocyte-derived and myeloid DC in whole human blood in a time- and dose-dependent manner. DC preconditioning with rapamycin-loaded nanoparticles, resulted in a maturation resistant phenotype and significantly suppressed allogeneic T-cell proliferation.
Keywords: Dendritic cells; immunomodulation; nanomedicine; nanoparticles; porous silicon; rapamycin; targeting
Rights: © 2017 Elsevier Ltd. All rights reserved.
RMID: 0030078475
DOI: 10.1016/j.biomaterials.2017.11.017
Appears in Collections:Medicine publications

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