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Type: Journal article
Title: Formulation optimization of chitosan-stabilized silver nanoparticles using in vitro antimicrobial assay
Author: Pansara, C.
Chan, W.
Parikh, A.
Trott, D.
Mehta, T.
Mishra, R.
Garg, S.
Citation: Journal of Pharmaceutical Sciences, 2019; 108(2):1007-1016
Publisher: Elsevier
Issue Date: 2019
ISSN: 0022-3549
Statement of
Chintan Pansara, Wei Yee Chan, Ankit Parikh, Darren J. Trott, Tejal Mehta, Renuka Mishra, Sanjay Garg
Abstract: Antimicrobial resistance at the infected site is a serious medical issue that increases patient morbidity and mortality. Silver has antibacterial activity associated with some dose-dependent toxicity. Silver nanoparticles, due to larger surface area, have antibacterial properties, which make them useful in the treatment of infections. Chitosan-stabilized silver nanoparticles (CH-AgNP) were formulated and evaluated for minimal inhibitory concentration and minimal bactericidal concentration testing against Staphylococcus aureus ATCC 29213, S aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and 20 methicillin-resistant S aureus isolates. Minimum biofilm eradication concentration study was used to evaluate the biofilm reduction, and in vitro antimicrobial checkerboard assays were performed. The effective optimum ratio of AgNP:chitosan solution was 1:4. Minimal inhibitory concentration and minimal bactericidal concentration ranges of CH-AgNP were 4 to 14 times lower compared to AgNP alone against methicillin-resistant S aureus isolates. Minimum biofilm eradication concentration values of CH-AgNP for ATCC PA-01, P aeruginosa isolate 1, and P aeruginosa isolate 2 were found to be >84.59 μg/mL, 42.29 μg/mL, and 21.15 μg/mL, respectively. Thus, CH-AgNP is a potential formulation for wound treatment and management of infected sites associated with antimicrobial resistance.
Keywords: Chitosan; silver; chitosan-silver nanoparticles; antibacterial activity; minimum inhibitory concentration; minimum bactericidal activity; biofilm minimum biofilm eradication concentration assay
Rights: © 2019 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.
DOI: 10.1016/j.xphs.2018.09.011
Appears in Collections:Animal and Veterinary Sciences publications
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