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Type: Journal article
Title: MyD88 is a critical regulator of hematopoietic cell-mediated neuroprotection seen after stroke
Author: Downes, C.
Wong, C.
Henley, K.
Guio-Aguilar, P.
Zhang, M.
Ates, R.
Mansell, A.
Kile, B.
Crack, P.
Citation: PLoS One, 2013; 8(3):e57948-e57948
Publisher: Public Library of Science
Issue Date: 2013
ISSN: 1932-6203
Statement of
Catherine E. Downes, Connie H. Y. Wong, Katya J. Henley, Pedro L. Guio-Aguilar, Moses Zhang, Robert Ates, Ashley Mansell, Benjamin T. Kile, Peter J. Crack
Abstract: Neuroinflammation is critical in the neural cell death seen in stroke. It has been shown that CNS and peripheral responses drive this neuroinflammatory response in the brain. The Toll-like receptors (TLRs) are important regulators of inflammation in response to both exogenous and endogenous stressors. Taking advantage of a downstream adapter molecule that controls the majority of TLR signalling, this study investigated the role of the TLR adaptor protein myeloid differentiation factor 88 (MyD88) in the control of CNS and peripheral inflammation. Reversible middle-cerebral artery occlusion was used as the model of stroke in vivo; in vitro primary cultured neurons and glia were subject to four hours of oxygen and glucose deprivation (OGD). Both in vitro and in vivo Myd88(-/-) animals or cells were compared with wild type (WT). We found that after stroke Myd88(-/-) animals have a larger infarct volume compared to WT animals. Interestingly, in vitro there was no difference between the survival of Myd88(-/-) and WT cells following OGD, suggesting that peripheral responses were influencing stroke outcome. We therefore generated bone marrow chimeras and found that Myd88(-/-) animals have a smaller stroke infarct than their radiation naive counterparts if their hematopoietic cells are WT. Furthermore, WT animals have a larger stroke than their radiation naive counterparts if the hematopoietic cells are Myd88(-/-) . We have demonstrated that MyD88-dependent signalling in the hematopoietic cell lineage reduces infarct size following stroke and that infiltrating cells to the site of neuroinflammation are neuroprotective following stroke.
Keywords: Mice, Inbred C57BL
Rights: © 2013 Downes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0057948
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