Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/121942
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Type: | Journal article |
Title: | A sustainable biomineralization approach for the synthesis of highly fluorescent ultra-small pt nanoclusters |
Author: | Balu, R. Knott, R. Elvin, C.M. Hill, A.J. R Choudhury, N. Dutta, N.K. |
Citation: | Biosensors, 2019; 9(4):1-13 |
Publisher: | MDPI |
Issue Date: | 2019 |
ISSN: | 2079-6374 2079-6374 |
Statement of Responsibility: | Rajkamal Balu, Robert Knott, Christopher M. Elvin, Anita J. Hill, Namita R. Choudhury and Naba K. Dutta |
Abstract: | Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed. |
Keywords: | Quantum dot; noble metal clusters; fluorescent platinum nanoclusters; biosensors; biomineralization; intrinsically disordered protein; protein polymer; small angle X-ray scattering |
Rights: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/bios9040128 |
Grant ID: | http://purl.org/au-research/grants/arc/Discovery http://purl.org/au-research/grants/arc/DP120103537 |
Published version: | http://dx.doi.org/10.3390/bios9040128 |
Appears in Collections: | Aurora harvest 4 Chemical Engineering publications |
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hdl_121942.pdf | Published version | 1.99 MB | Adobe PDF | View/Open |
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