Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/122027
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dc.contributor.authorEisenhofer, R.-
dc.contributor.authorMinich, J.-
dc.contributor.authorMarotz, C.-
dc.contributor.authorCooper, A.-
dc.contributor.authorKnight, R.-
dc.contributor.authorWeyrich, L.-
dc.date.issued2019-
dc.identifier.citationTrends in Microbiology, 2019; 27(2):105-117-
dc.identifier.issn0966-842X-
dc.identifier.issn1878-4380-
dc.identifier.urihttp://hdl.handle.net/2440/122027-
dc.description.abstractNext-generation sequencing approaches in microbiome research have allowed surveys of microbial communities, their genomes, and their functions with higher sensitivity than ever before. However, this sensitivity is a double-edged sword because these tools also efficiently detect contaminant DNA and cross-contamination, which can confound the interpretation of microbiome data. Therefore, there is an urgent need to integrate key controls into microbiome research to improve the integrity of microbiome studies. Here, we review how contaminant DNA and cross-contamination arise within microbiome studies and discuss their negative impacts, especially during the analysis of low microbial biomass samples. We then identify several key measures that researchers can implement to reduce the impact of contaminant DNA and cross-contamination during microbiome research. We put forward a set of minimal experimental criteria, the 'RIDE' checklist, to improve the validity of future low microbial biomass research.-
dc.description.statementofresponsibilityRaphael Eisenhofer, Jeremiah J. Minich, Clarisse Marotz, Alan Cooper, Rob Knight, and Laura S. Weyrich-
dc.language.isoen-
dc.publisherElsevier; Cell Press-
dc.rights© 2018 Elsevier Ltd. All rights reserved.-
dc.subjectMicrobiome; microbiota; DNA contamination; laboratory contamination; low biomass; methodology-
dc.titleContamination in low microbial biomass microbiome studies: issues and recommendations-
dc.typeJournal article-
dc.identifier.doi10.1016/j.tim.2018.11.003-
dc.relation.granthttp://purl.org/au-research/grants/arc/DE150101574-
dc.relation.granthttp://purl.org/au-research/grants/arc/CE170100015-
pubs.publication-statusPublished-
dc.identifier.orcidCooper, A. [0000-0002-7738-7851]-
Appears in Collections:Aurora harvest 4
Australian Centre for Ancient DNA publications

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