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Type: Journal article
Title: Use of FDA and flow cytometry to assess metabolic activity as an indicator of nutrient status in phytoplankton
Author: Brookes, J.
Geary, S.
Ganf, G.
Burch, M.
Citation: Marine and Freshwater Research, 2000; 51(8):817-823
Publisher: C S I R O Publishing
Issue Date: 2000
ISSN: 1323-1650
Abstract: This study reports the use of a technique to determine nutrient limitation of cultured and natural phytoplankton. The technique, an FDA-activity assay, which is usually used to assess cell viability, was used to measure metabolic activity in response to nutrient addition; the metabolic activity of phytoplankton was determined as the rate of hydrolysis of fluorescein diacetate (FDA), by intracellular esterases, to fluorescein, which was detectedusing a flow cytometer. Replacement of the limiting nutrient to nitrogen- or phosphorus-limited cultures and field populations resulted in an increase in metabolic activity that was detectable 24 h after nutrient addition. By flow cytometry, the natural phytoplankton community can be divided into different taxonomic groups; the response of these to FDA could be determined individually to allow identification of the nutrients limiting each type of phytoplankton. This would be more specific than the assessment of a whole-community response, which may mask subtle differences among taxa.
Description: © CSIRO 2000
RMID: 0001000005
DOI: 10.1071/MF00048
Appears in Collections:Ecology, Evolution and Landscape Science publications
Environment Institute Leaders publications
Earth and Environmental Sciences publications
Earth and Environmental Sciences publications

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