Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/122539
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Type: Journal article
Title: A paradigm in immunochemistry, revealed by monoclonal antibodies to spatially distinct epitopes on Syntenin-1
Author: Johnson, I.R.D.
Sorvina, A.
Logan, J.M.
Moore, C.R.
Heatlie, J.K.
Parkinson-Lawrence, E.J.
Selemidis, S.
O'Leary, J.J.
Butler, L.M.
Brooks, D.A.
Citation: International Journal of Molecular Sciences, 2019; 20(23):6035-1-6035-16
Publisher: MDPI
Issue Date: 2019
ISSN: 1422-0067
1422-0067
Statement of
Responsibility: 
Ian R. D. Johnson, Alexandra Sorvina, Jessica M. Logan, Courtney R. Moore, Jessica K. Heatlie, Emma J. Parkinson-Lawrence, Stavros Selemidis, John J. O’Leary, Lisa M. Butler and Douglas A. Brooks
Abstract: Syntenin-1 is an essential multi-functional adaptor protein, which has multiple roles in membrane trafficking and exosome biogenesis, as well as scaffolding interactions with either the actin cytoskeleton or focal adhesions. However, how this functional multiplicity relates to syntenin-1 distribution in different endosome compartments or other intracellular locations and its underlying involvement in cancer pathogenesis have yet to be fully defined. To help facilitate the investigation of syntenin-1 biology, we developed two specific monoclonal antibodies (Synt-2C6 and Synt-3A11) to spatially distinct linear sequence epitopes on syntenin-1, which were each designed to be unique at the six-amino acid level. These antibodies produced very different intracellular staining patterns, with Synt-2C6 detecting endosomes and Synt-3A11 producing a fibrillar staining pattern suggesting a cytoskeletal localisation. Treatment of cells with Nocodazole altered the intracellular localisation of Synt-3A11, which was consistent with the syntenin-1 protein interacting with microtubules. In prostate tissue biopsies, Synt-3A11 defined atrophy and early-stage prostate cancer, whereas Synt-2C6 only showed minimal interaction with atrophic tissue. This highlights a critical need for site-specific antibodies and a knowledge of their reactivity to define differential protein distributions, interactions and functions, which may differ between normal and malignant cells.
Keywords: endosomes; microtubules; prostate cancer; syntenin-1
Rights: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
RMID: 1000009649
DOI: 10.3390/ijms20236035
Grant ID: http://purl.org/au-research/grants/arc/FT130101004
http://purl.org/au-research/grants/nhmrc/1092904
Appears in Collections:Medicine publications

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